Developmentally excised DNA sequences in Euplotes crassus capable of forming G quartets.
Developmentally excised DNA sequences in Euplotes crassus capable of forming G quartets.
复制标题
克拉苏幼虫中发育性切除的 DNA 序列能够形成 G 四联体。
DOI：
10.1073/pnas.92.6.1979
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发表时间：
1995
影响因子：
11.1
通讯作者：
Klobutcher,LA
中科院分区：
文献类型：
--
作者：
Klobutcher,LA
在下毛纤毛虫原生动物Crassus中，在形成新的大核过程中，数以万计的DNA片段被DNA断裂和重新连接事件消除。这项研究提供了一类被称为端粒重复类内部消除序列(TELES)的消除序列的证据。TELES比大多数以前描述的IESS都要短，它们的DNA序列与生物体的端粒重复序列相似。TELES在染色体碎裂/端粒增加的发育期被切除，这比以前描述的Less要晚。更多的研究表明，代表TELES的寡核苷酸就像端粒重复序列一样，能够在体外形成G-四元组结构。
Tens of thousands of DNA segments are eliminated by DNA breakage and rejoining events during the formation of a new macronucleus in the hypotrichous ciliated protozoan Euplotes crassus. This study presents evidence for a class of eliminated sequences referred to as telomeric-repeat-like internal eliminated sequences (TelIESs). TelIESs are shorter (< 50 bp) than most previously characterized IESs and their DNA sequences resemble the telomeric repeat sequences of the organism. The TelIESs are excised during the developmental period of chromosome fragmentation/telomere addition, which is later than previously characterized IESs. Additional studies demonstrate that oligonucleotides representing the TelIESs are, like telomeric repeats, capable of forming G-quartet structures in vitro.