Retinol-binding protein 4 in adipose and placental tissue of women with gestational diabetes
Retinol-binding protein 4 in adipose and placental tissue of women with gestational diabetes
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DOI：
10.3109/09513590.2011.579651
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发表时间：
2011-12-01
影响因子：
2
通讯作者：
Szamatowicz, Jacek
中科院分区：
文献类型：
--
作者：
Kuzmicki, Mariusz;Telejko, Beata;Szamatowicz, Jacek
在本研究中，我们评估了血清中的视黄醇结合蛋白4（RBP4）的水平和表达的RBP4，葡萄糖转运蛋白4（GLUT 4）和过氧化物酶体增殖物激活受体γ（PPAR γ）的mRNA（采用定量真实的时间PCR）在皮下脂肪组织（SAT），内脏脂肪组织（VAT）和胎盘组织获得妊娠糖尿病（GDM）和健康孕妇。血清RBP4浓度及其在SAT中的表达在GDM女性中高于对照组（p = 0.03）。血清或组织RBP4与胰岛素抵抗指数之间无相关性。GDM组血清RBP4水平与SAT中RBP4 mRNA表达水平相关（r = 0.67，p = 0.007）。逐步回归分析显示，SAT中RBP4 mRNA的表达是由GLUT4 mRNA表达独立预测的（β = 0.59，p = 0.003）和GDM的存在（β = 0.46，p = 0.01），而VAT中RBP4 mRNA表达与PPARg mRNA表达相关（β = 0.64，p = 0.0003）和患者年龄（β = 70.38，p = 0.03）。总之，我们的研究结果表明，SAT中RBP4的表达升高可能有助于GDM受试者循环RBP4的增加。
In the present study, we evaluated serum levels of retinol-binding protein 4 (RBP4) and the expression of RBP4, glucose transporter-4 (GLUT4) and peroxisome proliferator activated receptor gamma (PPAR gamma) mRNA (using quantitative real time-PCR) in subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT) and placental tissue obtained from patients with gestational diabetes (GDM) and healthy pregnant women. Serum RBP4 concentrations and its expression in SAT were higher in the women with GDM than in the controls (p = 0.03). No association between serum or tissue RBP4 and the indices of insulin resistance was noted. In the GDM group serum RBP4 correlated with its mRNA expression in SAT (r = 0.67, p = 0.007). Stepwise regression analysis revealed that RBP4 mRNA expression in SAT was independently predicted by GLUT4 mRNA expression (beta = 0.59, p = 0.003) and the presence of GDM (beta = 0.46, p = 0.01), whereas RBP4 mRNA expression in VAT was related to PPARg mRNA expression (beta = 0.64, p = 0.0003) and the patient's age (beta = 70.38, p = 0.03). In conclusion, our results suggest that the elevated expression of RBP4 in SAT may contribute to the increase in circulating RBP4 in GDM subjects.