Chemical modification of actin. Acceleration of polymerization and reduction of network formation by reaction with N-ethylmaleimide, (iodoacetamido)tetramethylrhodamine, or 7-chloro-4-nitro-2,1,3-benzoxadiazole.
Chemical modification of actin. Acceleration of polymerization and reduction of network formation by reaction with N-ethylmaleimide, (iodoacetamido)tetramethylrhodamine, or 7-chloro-4-nitro-2,1,3-benzoxadiazole.
复制标题
肌动蛋白的化学修饰。
DOI：
10.1021/bi00267a004
复制
发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Frieden,C
中科院分区：
文献类型：
--
作者：
Tait,JF;Frieden,C
Jonathan F.Tail和Carl Frieden*摘要：我们研究了兔骨骼肌肌动蛋白在Cys-373位用四乙基马来酰亚胺或(碘乙酰胺)四甲基罗丹明标记，以及A‘-乙基雄酰亚胺-肌动蛋白进一步用7-氯-4-硝基-2，1，3-苯并恶二唑(主要标记Lys-372)修饰的性质。这三种衍生物的聚合速度都比未标记的肌动蛋白快。根据荧光漂白恢复和低剪切粘度计的测量，与天然肌动蛋白相比，这三种蛋白都显示出较低的网络形成程度。A-乙基马来酰亚胺
Jonathan F. Tail and Carl Frieden* abstract: We examined the properties of rabbitskeletal muscle actin labeled at Cys-373 with IV-ethylmaleimide or with (iodoacetamido) tetramethylrhodamine, and of A’-ethylmale-imide-actin further modified with 7-chloro-4-nitro-2, 1, 3-benzoxadiazole (which primarily labels Lys-372). All three derivatives polymerize more rapidly than unlabeled actin. As measured by fluorescence photobleaching recovery and low-shear viscometry, all three also show a lower extent of network formation relative to native actin. A-Ethylmaleimide has a