Inflammasome activation and vitiligo/nonsegmental vitiligo progression
Inflammasome activation and vitiligo/nonsegmental vitiligo progression
复制标题
DOI：
10.1111/bjd.12691
复制
发表时间：
2014-04-01
影响因子：
10.3
通讯作者：
Cario-Andre, M.
中科院分区：
文献类型：
--
作者：
Marie, J.;Kovacs, D.;Cario-Andre, M.
在白癜风/非节段性白癜风（NSV）患者中发现NLR（核苷酸结合域和富含亮氨酸重复序列）家族、pyrin结构域含蛋白1 （NLRP1）的多态性，并且在病变皮肤活检的前沿检测到NLRP1的表达增加。目的探讨NLRP1免疫染色在白癜风/NSV患者皮损及皮损周围皮肤中的存在及强度，并探讨NLRP1与白细胞介素(IL)-1 β表达、淋巴细胞浸润及疾病活动性之间可能存在的相关性。方法连续14例白癜风/NSV患者中，8例为活动性疾病[白癜风欧洲工作组（VETF）传播评分+ 1 ~ + 5]，1例病情稳定，5例病情退行性疾病（VETF传播评分- 1 ~ - 3）。我们对白癜风皮肤进行NLRP1、B和T淋巴细胞、IL-1 β和钾化因子7的免疫染色。结果NLRP1和IL-1 β免疫染色与白癜风/NSV皮肤的进展性相关（P值分别为0.009和0.04），且优于单纯检测淋巴细胞浸润。结论：除了检测白癜风/非NSV病变进展边缘的炎症浸润外，NLRP1炎症小体的标志物可能是评估疾病活动性的有用测试。
Background Polymorphisms of NLR (nucleotide-binding domain and leucine rich repeat containing) family, pyrin domain containing protein 1 (NLRP1) have been found in patients with vitiligo/nonsegmental vitiligo (NSV), and increased NLRP1 expression has been detected in the leading edge of lesional skin biopsies. Objectives To evaluate the presence and intensity of NLRP1 immunostaining in lesional and perilesional skin of patients with vitiligo/NSV and to search for possible correlations between NLRP1 and interleukin (IL)-1 beta expression, lymphocytic infiltrates and disease activity.Methods Of 14 consecutive vitiligo/NSV patients, eight had active disease [Vitiligo European Task Force (VETF) spreading score + 1 to + 5], one patient had stable disease and five patients had regressive disease (VETF spreading score - 1 to - 3). We performed immunostaining for NLRP1, B and T lymphocytes, IL-1 beta and kallikrein 7 on lesional and perilesional vitiligo skin.Results NLRP1 and IL-1 beta immunostaining in perilesional vitiligo/NSV skin was significantly associated with progressive disease (P = 0.009 and 0.04, respectively) and performed better than the simple detection of lymphocytic infiltrates.Conclusions Our findings suggest that markers of the NLRP1 inflammasome could be a useful test for assessing disease activity in addition to the detection of inflammatory infiltrates in the progressing margins of vitiligo/ NSV lesions.