Passive protection of purified yolk immunoglobulin administered against Shiga toxin 1 in mouse models.
Passive protection of purified yolk immunoglobulin administered against Shiga toxin 1 in mouse models.
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DOI：
10.1139/w10-087
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发表时间：
2010-11
影响因子：
2.8
通讯作者：
Qin Wang;X. Hou;Kun Cai;Tao Li;Yue Liu;Wei Tu;Le Xiao;Shi-zhong Bao;Jing Shi;Xiang Gao;H. Liu;Renmao Tian;Hui Wang
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文献类型：
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作者：
Qin Wang;X. Hou;Kun Cai;Tao Li;Yue Liu;Wei Tu;Le Xiao;Shi-zhong Bao;Jing Shi;Xiang Gao;H. Liu;Renmao Tian;Hui Wang
由大肠杆菌O 157：H7产生的滋贺毒素可引起多种肠道疾病，如致死性出血性结肠炎和溶血性尿毒症综合征。本研究利用大肠杆菌表达滋贺毒素1型（Stx 1）的B亚单位蛋白。coli系统，经Ni柱亲和层析纯化，并以此为免疫原免疫产蛋鸡，制备卵黄免疫球蛋白（IgY）。IgY的滴度随着加强接种而逐渐增加，最终达到105的水平，在1年内保持稳定。通过体外和体内实验评价IgY对Stx 1的保护作用。结果表明，抗Stx 1 IgY能有效地阻断Stx 1与Hela细胞的结合，对毒素攻击的BALB/c小鼠具有保护作用。这些数据表明，在滋贺毒素中毒的情况下，使用蛋黄IgY作为治疗干预的设施。
Shiga toxins produced by Escherichia coli O157:H7 cause a wide spectrum of enteric diseases, such as lethal hemorrhagic colitis and hemolytic uremic syndrome. In this study, the B subunit protein of Shiga toxin type 1 (Stx1) was produced in the E. coli system, was further purified by Ni-column Affinity Chromatography method, and was then used as an immunogen to immunize laying hens for yolk immunoglobulin (IgY) production. Titers of IgY increased gradually with boosting vaccination and, finally, reached a level of 105, remaining steady over 1 year. Then the protective efficacy of IgY against Stx1 was evaluated by in vitro and in vivo experiments. It was shown that the anti-Stx1 IgY could effectively block the binding of Stx1 to the Hela cells and could protect BALB/c mice from toxin challenges. The data indicates the facility of using egg yolk IgY as a therapeutic intervention in cases of Shiga toxin intoxication.