Effects of matrix macromolecules on chondrocyte gene expression: synthesis of a low molecular weight collagen species by cells cultured within collagen gels.
Effects of matrix macromolecules on chondrocyte gene expression: synthesis of a low molecular weight collagen species by cells cultured within collagen gels.
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基质大分子对软骨细胞基因表达的影响：通过在胶原蛋白内培养的细胞合成低分子量胶原蛋白物种。
DOI：
10.1083/jcb.93.3.767
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发表时间：
1982-06
影响因子：
7.8
通讯作者：
Grant, M E
中科院分区：
文献类型：
--
作者：
Gibson, G J;Schor, S L;Grant, M E
鸡胚胸骨软骨细胞在三维胶原凝胶中培养，作为细胞外基质大分子对软骨细胞基因表达影响研究的一部分。数据表明，软骨细胞培养在这样一个胶原环境中合成显着更多的迄今未确定的，低分子量的胶原蛋白种类比细胞生长在塑料组织培养皿中的常规方式。这种低分子量胶原物质含有非胶原结构域（如胃蛋白酶轻度消化后其分子大小减小所示），与已知的胶原类型不同（如CNBr肽分析所判断），并形成软骨细胞产生的不溶性胶原基质的一部分。在凝胶内生长的细胞倾向于形成由线性排列的细胞组成的集落，这让人联想到生长软骨中的细胞组织。
Chick-embryo sternal chondrocytes have been cultured within three- dimensional collagen gels as part of a study concerned with the effects of extracellular matrix macromolecules on chondrocyte gene expression. Data are presented indicating that chondrocytes cultured within such a collagenous environment synthesize significantly more of an hitherto unidentified, low molecular weight collagen species than do cells grown on plastic tissue-culture dishes in the conventional manner. This low molecular weight collagen species contains noncollagenous domains (as indicated by its decreased molecular size after mild pepsin digestion), is distinct from the known collagen types (as judged by CNBr peptide analysis), and forms part of the insoluble collagenous matrix produced by the chondrocytes. Cells growing within the gel tend to form colonies consisting of a linear array of cells reminiscent of the cellular organization in growth cartilage.