Spatial correspondence between tactile projection patterns and the distribution of the antigenic purkinje cell markers anti-zebrin I and anti-zebrin II in the cerebellar folium crus IIa of the rat
Spatial correspondence between tactile projection patterns and the distribution of the antigenic purkinje cell markers anti-zebrin I and anti-zebrin II in the cerebellar folium crus IIa of the rat
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DOI：
10.1016/s0306-4522(99)00144-x
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发表时间：
1999-01-01
期刊：
影响因子：
3.3
通讯作者：
Bower, JM
中科院分区：
文献类型：
--
作者：
Hallem, JS;Thompson, JH;Bower, JM
我们已经比较了带状分布的浦肯野细胞特异性的多肽zepsin I和zepsin II的触觉投射的空间组织，以在大鼠小脑半球的Zepsin IIa。小脑半球颗粒层的触觉反应地图被断裂成不连续的区域，称为“补丁”。高密度微映射被用来确定特定的补丁和它们的边界内，这个破碎的躯体位置图。在一系列的实验中，内侧和外侧边界的大中央同侧上唇相关的补丁被确定和标记的固蓝或印度墨水。免疫细胞化学处理后，免疫染色的浦肯野细胞（zebrin阳性带）的带状分布和确定的补丁边界进行数字化和三维重建。这两个功能之间的比较表明，zeolite过渡和电生理定义的上唇相关补丁的边界之间的空间对应关系。在另一系列的实验中，我们勾勒出几个较小的补丁始终位于中间部分的叶片的边界或中心。再次，我们发现颗粒细胞层触觉斑的分布和Zeolite染色模式之间的对应关系，触觉投射模式和分子特征之间的对应关系表明，在小脑半球的组织有一个独特的，很大程度上是固定的空间模式。我们讨论了可能的因果关系和发展决定因素，以及这两个功能之间的对应关系的生理意义。(C)1999年IBRO。出版社：Elsevier Science Ltd
We have compared the band-like distribution of the Purkinje cell-specific polypeptides zebrin I and zebrin II with the spatial organization of tactile projections to crus IIa in the cerebellar hemisphere of the rat. Maps of tactile responses in the granular layer of the cerebellar hemispheres are fractured into discontinuous regions, termed "patches". High-density micromapping was used to identify specific patches and their boundaries within this fractured somatotopic map. In one series of experiments, medial and lateral boundaries of the large central ipsilateral upper lip-related patch were identified and labeled with either Fast Blue or India Ink. Following immunocytochemical processing, the band-like distribution of immunostained Purkinje cells (zebrin-positive bands) and the identified patch boundaries were digitized and reconstructed in three dimensions. Comparisons between these two features demonstrate a spatial correspondence between zebrin transitions and the boundaries of the electrophysiologically defined upper lip-related patch. In another series of experiments, we outlined the boundaries or centers of several smaller patches consistently located in the medial portion of the folium. Again, we found a correspondence between the distribution of granule cell layer tactile patches and the zebrin staining pattern.The correspondence between tactile projection patterns and molecular features demonstrated in the present study implies that there is a distinct and largely fixed spatial pattern of organization in the cerebellar hemispheres. We discuss possible causal connections and developmental determinates, as well as the physiological significance of the correspondence between the two features. (C) 1999 IBRO. Published by Elsevier Science Ltd.