Identification of natural sources of resistance to bipartite begomovirus TYLCKaV in Solanum melongena
Identification of natural sources of resistance to bipartite begomovirus TYLCKaV in Solanum melongena
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茄属二分叶病毒 TYLCKaV 天然抗性来源的鉴定
DOI：
10.1007/s10681-023-03180-7
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发表时间：
2023
期刊：
影响因子：
1.9
通讯作者：
Koeda Sota
中科院分区：
文献类型：
--
作者：
Kikkawa Kyohei;Tanaka Minami;Kesumawati Elly;Koeda Sota
由菜豆花叶病毒引起的黄化曲叶病已成为世界上几个地区茄子（Solanummelongena）生产的主要限制因素。番茄黄曲叶坎恰纳布里病毒（TYLCKaV）是一种从印度支那半岛、华南和东南亚沿海地区患病茄子中分离出的双分菜豆病毒。在这项研究中，5个菜豆花叶病毒种分离在印度尼西亚，包括TYLCKaV，接种茄子使用感染性克隆。在接种的菜豆花叶病毒中，TYLCKaV单独诱导茄子典型的黄化和卷曲症状。将TYLCKaV接种到印度尼西亚常见的一组农作物物种（茄子、番茄、辣椒、烟草、黄瓜、甜瓜和南瓜）中，澄清了TYLCKaV主要在茄子和番茄中感染并引起黄化曲叶病。将TYLCKaV渗入736份茄子材料中，筛选出33个抗性候选材料。进一步通过TYLCKaV嫁接接种的抗性候选材料的评价澄清，10份材料被归类为中抗，23份材料为高抗。在高抗品种中，有22个品种的病毒DNA积累量明显低于感病对照。这22份高抗材料为茄子抗病毒育种提供了宝贵的遗传资源。
Yellow leaf curl disease caused by begomoviruses has emerged as a major limitation to the production of eggplant (Solanum melongena) in several regions of the world. Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) is a bipartite begomovirus isolated from diseased eggplant in the areas of the Indochinese peninsula, South China, and Maritime Southeast Asia. In this study, five begomovirus species isolated in Indonesia, including TYLCKaV, were inoculated to eggplant using infectious clones. Among the inoculated begomoviruses, TYLCKaV alone induced typical yellowing and curling symptoms in eggplant. Inoculation of TYLCKaV to a panel of crop species (eggplant, tomato, pepper, tobacco, cucumber, melon, and squash) commonly grown in Indonesia clarified that TYLCKaV infects and causes yellow leaf curl disease mainly in eggplant and tomato. Agroinfiltration of TYLCKaV to 736 accessions of eggplant enabled selection of 33 resistant candidates. Further evaluation by TYLCKaV graft-inoculation of the resistant candidates clarified that 10 accessions were classifiable as moderately resistant and 23 accessions as highly resistant. Among the highly resistant accessions, 22 accessions restricted viral DNA accumulation to a significantly lower amount than the susceptible control. These 22 highly resistant accessions represent valuable genetic resources for breeding begomovirus resistance in eggplant.