Isoprenylcysteine carboxy methylation is essential for development in Dictyostelium discoideum.
Isoprenylcysteine carboxy methylation is essential for development in Dictyostelium discoideum.
复制标题
异戊二烯半胱氨酸羧甲基化对于盘基网柄菌的发育至关重要。
DOI：
10.1091/mbc.e06-11-1006
复制
发表时间：
2007
影响因子：
3.3
通讯作者：
Cox,EdwardC
中科院分区：
文献类型：
--
作者：
Chen,Ying;McQuade,KyleJ;Guan,Xiao-Juan;Thomason,PeterA;Wert,MichaelS;Stock,JeffryB;Cox,EdwardC
小GTP酶的Ras超家族成员和异源三聚体G蛋白γ亚基通过异戊二烯基半胱氨酸甲基转移酶在其羧基末端半胱氨酸残基上甲基化。在盘基网柄藻中，cAMP刺激饥饿细胞后数秒内发生小的GT3甲基化，并迅速恢复到基础水平，表明在cAMP依赖性信号传导中起重要作用。删除异戊二烯半胱氨酸甲基转移酶编码基因会导致严重的缺陷。饥饿的突变细胞不以持续的方式传播cAMP波，并且它们不聚集。当细胞用外源性cAMP脉冲或与野生型细胞共接种时，运动性被拯救，但被拯救的细胞表现出改变的极性。已经聚集的cAMP脉冲甲基转移酶缺陷细胞不能分化，但是在野生型背景中接种的突变细胞能够分化。RasG的定位和信号传导在突变体中改变。异源三聚体G γ蛋白亚基的定位是正常的，但在突变细胞中信号发生了改变。这些数据表明异戊二烯基半胱氨酸甲基化是细胞间信号传导和发育所必需的。
Members of the Ras superfamily of small GTPases and the heterotrimeric G protein γ subunit are methylated on their carboxy-terminal cysteine residues by isoprenylcysteine methyltransferase. InDictyostelium discoideum, small GTPase methylation occurs seconds after stimulation of starving cells by cAMP and returns quickly to basal levels, suggesting an important role in cAMP-dependent signaling. Deleting the isoprenylcysteine methyltransferase-encoding gene causes dramatic defects. Starving mutant cells do not propagate cAMP waves in a sustained manner, and they do not aggregate. Motility is rescued when cells are pulsed with exogenous cAMP, or coplated with wild-type cells, but the rescued cells exhibit altered polarity. cAMP-pulsed methyltransferase-deficient cells that have aggregated fail to differentiate, but mutant cells plated in a wild-type background are able to do so. Localization of and signaling by RasG is altered in the mutant. Localization of the heterotrimeric Gγ protein subunit was normal, but signaling was altered in mutant cells. These data indicate that isoprenylcysteine methylation is required for intercellular signaling and development inDictyostelium.