Establishment of primary cell cultures from fish calcified tissues
Establishment of primary cell cultures from fish calcified tissues
复制标题
鱼钙化组织原代细胞培养物的建立
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
V. Laizé
中科院分区：
文献类型：
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作者：
Cátia L. Marques;Marta S. Rafael;M. Cancela;V. Laizé
鱼类是研究脊椎动物骨骼发育和组织矿化等生理过程的理想模式生物。然而，缺乏良好表征的源自鱼钙化组织的体外细胞系统。我们在这里描述了一个协议，成功地用于开发第一个钙化组织来源的细胞培养的鱼源。从年轻的金头鲷收集的脊椎和鳃弓被破碎，然后提交胶原酶和胰蛋白酶的联合作用，有效地释放嵌入在胶原细胞外基质中的细胞。原代培养物保持在标准条件下，并自发转化形成连续的细胞系，适用于研究组织矿化机制的海鸟。通过针对每个特定情况调整参数，这种简单且廉价的方案也适用于其他钙化组织和物种。
Fishes have been recently recognized as a suitable model organism to study vertebrate physiological processes, in particular skeletal development and tissue mineralization. However, there is a lack of well characterized in vitro cell systems derived from fish calcified tissues. We describe here a protocol that was successfully used to develop the first calcified tissue-derived cell cultures of fish origin. Vertebra and branchial arches collected from young gilthead seabreams were fragmented then submitted to the combined action of collagenase and trypsin to efficiently release cells embedded in the collagenous extracellular matrix. Primary cultures were maintained under standard conditions and spontaneously transformed to form continuous cell lines suitable for studying mechanisms of tissue mineralization in seabream. This simple and inexpensive protocol is also applicable to other calcified tissues and species by adjusting parameters to each particular case.
影响因子：
2.7
作者：
Fisher, S;Jagadeeswaran, P;Halpern, ME
通讯作者：
Halpern, ME