Gene expression profiling of the pre-implantation mouse embryo by microarray analysis: Comparison of the two-cell stage and two-cell block
Gene expression profiling of the pre-implantation mouse embryo by microarray analysis: Comparison of the two-cell stage and two-cell block
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DOI：
10.1016/j.theriogenology.2006.01.059
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发表时间：
2006-09-01
期刊：
影响因子：
2.8
通讯作者：
Cha, Kwang-Yul
中科院分区：
文献类型：
--
作者：
Jeong, Hye-Jin;Kim, Hyun Jae;Cha, Kwang-Yul
为了提高我们对早期胚胎发育的分子机制的理解，迫切需要进一步表征卵母细胞和胚胎中的基因活性。从两细胞到四细胞阶段的过渡在植入前胚胎发育中特别重要，因为它涉及转录重编程和细胞分化。在这项研究中，我们使用了7.4 K的cDNA微阵列筛选mRNA转录水平在植入前小鼠胚胎。实时PCR用于确认微阵列数据。我们分析了7410个基因，并确定了4562个在植入前胚胎中差异表达的基因。我们选择了总共248个基因，这些基因在功能上参与了两细胞和两细胞块胚胎的显著表达变化。在这些基因中，114个在两细胞胚胎中下调，其余的（n = 134）在两细胞胚胎中上调。本研究提供了大量的基因在植入前小鼠胚胎的发育图谱，特别强调在两细胞胚胎和两细胞块胚胎的基因表达。基于这些数据的进一步调查将提供更好地了解各种外部条件的影响，并可能有助于对其他哺乳动物物种（包括人类）的着床前发育进行比较分析。(c)2006年爱思唯尔公司All rights reserved.
To improve our understanding of the molecular mechanisms underlying early embryo development, further characterization of gene activity in oocytes and embryos is urgently required. The transition from the two-cell to four-cell stage is particularly important in pre-implantation embryonic development, as it involves transcriptional reprogramming and cellular differentiation. In this study, we used a 7.4 K cDNA microarray to screen mRNA transcript levels in the pre-implantation mouse embryo. Real-time PCR was used to confirm microarray data. We profiled 7410 genes and identified 4562 genes that were differentially expressed in the pre-implantation embryo. We selected a total of 248 genes with significant expression changes that are functionally involved in the two-cell and two-cell block embryo. Of these genes, 114 were down-regulated and the remainder (n = 134) were up-regulated in the two-cell embryo. This study provides a developmental map of a large number of genes in the pre-implantation mouse embryo with particular emphasis on gene expression in the two-cell embryo and two-cell block embryo. Further investigations based on this data will provide a better understanding of the effects of various external conditions and may facilitate comparative analysis of preimplantation development in other mammalian species, including human. (c) 2006 Elsevier Inc. All rights reserved.