Developmental expression of the Drosophila zeste gene and localization of zeste protein on polytene chromosomes.
Developmental expression of the Drosophila zeste gene and localization of zeste protein on polytene chromosomes.
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DOI：
10.1101/gad.2.12b.1839
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发表时间：
1988-12
影响因子：
10.5
通讯作者：
V. Pirrotta;Sharon E. Bickel;Celestina Mariani
中科院分区：
文献类型：
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作者：
V. Pirrotta;Sharon E. Bickel;Celestina Mariani
zeste基因的表达在果蝇的整个生命周期中是不同的。它的转录是最丰富的母体RNA，下降到非常低的水平，在幼虫生长，但再次上升，在晚三龄幼虫和蛹。使用含有zeste-lacZ基因的转座子，我们发现zeste在不同阶段的组织分布中存在相应的变化。几乎无处不在的zeste-lacZ基因的表达被发现在晚期胚胎和一龄幼虫，但几乎完全消失，除了在大脑和性腺的三龄幼虫。在化蛹前不久，在成虫盘、马氏管和唾液腺中表达再次上升，并且再次在蛹中变得几乎无处不在。zeste继续在成人脑和性腺中表达。我们构建了携带热休克启动子控制的zeste基因的果蝇，并研究了zeste蛋白在其多线染色体以及野生型果蝇中的分布。使用亲和纯化的抗zeste抗体，我们发现，野生型唾液腺染色体含有约60个强带的zeste免疫荧光在特定的细胞学位置。含有hs-zeste基因的幼虫经热诱导后，出现数百条带。这些结果表明，在不同的发展阶段，在各种各样的基因的表达zeste的参与。
The expression of the zeste gene varies through the life cycle of the fly. Its transcription is most abundant in maternal RNA, declines to very low levels during larval growth, but rises again in late third instar larvae and pupae. Using transposons containing a zeste-lacZ gene, we found a corresponding variation in the tissue distribution of zeste from stage to stage. Nearly ubiquitous expression of the zeste-lacZ gene is found in late embryos and first instar larvae, but disappears almost completely except in brain and gonads by third instar larva. Shortly before pupation expression rises again in imaginal discs, Malpighian tubules, and salivary glands and again becomes nearly ubiquitous in pupae. zeste continues to be expressed in adult brain and gonads. We constructed flies carrying a zeste gene controlled by the heat shock promoter and studied the distribution of zeste protein in their polytene chromosomes as well as those of wild-type flies. Using affinity-purified anti-zeste antibodies, we find that wild-type salivary gland chromosomes contain about 60 strong bands of zeste immunofluorescence at specific cytological locations. After heat induction of larvae containing the hs-zeste gene, many hundreds of bands appear. These results suggest the involvement of zeste in the expression of a wide variety of genes at different developmental stages.