Capillary Growth of Rat Aortic Segments Cultured in Collagen Gel without Serum
Capillary Growth of Rat Aortic Segments Cultured in Collagen Gel without Serum
复制标题
无血清胶原凝胶培养大鼠主动脉节段的毛细血管生长
DOI：
10.1111/j.1440-1827.1989.tb02419.x
复制
发表时间：
1989
影响因子：
2.2
通讯作者：
M. Awai
中科院分区：
文献类型：
--
作者：
S. Kawasaki;M. Mori;M. Awai
在无血清条件下，利用大鼠主动脉节段的三维胶原凝胶研究血管生成过程。在无血清和无生长因子条件下，毛细血管形成网状结构和管状结构，内皮细胞产生von Willebrand因子、层粘连蛋白和IV型胶原，但与含20%胎牛血清(FCS)的培养液相比，毛细血管数量减少，生长缓慢。溴脱氧尿苷(BrdU)的掺入和羟基脲的生长抑制表明，毛细血管的生长主要依赖于细胞的增殖，而不是迁移。PRMI 1640和DM EM中的毛细血管生长相似，但比MEM中的毛细血管生长更有效。在Medium 199和Ham-F12中仅有轻微的增长。在培养液中加入血清可促进毛细血管的生长，与加入的量成正比。在无血清条件下，ITS(+)和成纤维细胞生长因子(成纤维细胞生长因子)促进毛细血管生长，但促进程度不明显。所使用的凝胶基质(I型胶原、I+11型胶原、IIV型胶原、纤维蛋白和血浆凝块)在毛细血管生长方面没有差异。
The process of angiogenesis was studied under serum‐free conditions using rat aortic segments in three‐dimensional collagen gel. In serum‐free and growth factor free conditions, the capillaries formed networks and tube‐like structures, and the endothelial cells produced von Willebrand factor, laminin and type IV collagen, but the number of capillaries was lower and their growth was slower than in medium containing 20% fetal calf serum (FCS). Incorporation of bromodeoxyuridine (BrdU) and inhibition of growth by hydroxyurea suggested that capillary growth depended mainly on cell proliferation and not on migration. Capillary growth in PRMI 1640 or DM EM was similar and more efficient than in MEM. Only slight growth was seen in Medium 199 and HAM‐F12. The addition of serum to the medium accelerated capillary growth in proportion to the amount added. In serum‐free conditions, ITS(+) and fibroblast growth factor (FGF) promoted capillary growth, but not to a significant extent. There ware no differences in capillary growth among the gel matrices used (type I collagen, type I+ 11 collagen, type I IV collagen, fibrin and plasma clot).