The role of Teashirt in proximal leg development in Drosophila:: Ectopic teashirt expression reveals different cell behaviours in ventral and dorsal domains
The role of Teashirt in proximal leg development in Drosophila:: Ectopic teashirt expression reveals different cell behaviours in ventral and dorsal domains
复制标题
DOI：
10.1006/dbio.1999.9452
复制
发表时间：
1999-11-15
影响因子：
2.7
通讯作者：
Kerridge, S
中科院分区：
文献类型：
--
作者：
Erkner, A;Gallet, A;Kerridge, S
本地化的转录因子指定的身份发展领域。在这里，我们分析的Teashirt锌指蛋白，这是表达在近端域的果蝇腿的功能。通过teashirt转基因的异位表达，我们表明Teashirt有助于近端和远端腿细胞之间的细胞-细胞粘附的差异。尽管表达Teashirt转基因的细胞克隆在内源性Teashirt结构域中存活，但在异位远端位置表达Teashirt的大多数细胞从上皮中丢失。在克隆恢复在远端域中，不同的效果被认为是依赖于相对于背腹轴的位置。在腹侧区域，无翼是信号，幸存的克隆表达Teashirt，并导致成人腿的异常。相反，侧克隆和背克隆通常不会积累Teashirt，对图案形成没有影响。一个例外的差分背腹效应发生在Teashirt表达和非表达细胞之间的边界。无论是异位和hypomorphic损失teashirt影响图案在所有位置的边界，这表明Teashirt在边界形成中起着至关重要的作用。讨论了转录和转录后机制在果蝇腿近端-远端轴模式中的作用。(C)北京：科学出版社.
Localised transcription factors specify the identity of developmental domains. Here we analyse the function of the Teashirt zinc finger protein, which is expressed in the proximal domain of the Drosophila leg. By ectopic expression of a teashirt transgene we show that Teashirt contributes to the differences in cell-cell adhesion between proximal and distal leg cells. Whereas clones of cells expressing the teashirt transgene survive in the endogenous Teashirt domain, most cells expressing Teashirt in an ectopic distal position are lost from the epithelium. In clones which were recovered in the distal domain, different effects were seen dependent on position with respect to the dorsal-ventral axis. In the ventral region, where Wingless is signalling, surviving clones express Teashirt and cause abnormalities in the adult leg. Contrarily, lateral and dorsal clones generally do not accumulate Teashirt and have no effect on patterning. One exception to the differential dorsal-ventral effects occurs at the boundary between Teashirt-expressing and -nonexpressing cells. Both ectopic and hypomorphic loss of teashirt affects patterning at all positions at the boundary, suggesting that Teashirt plays a crucial role in boundary formation. The results are discussed with respect to the roles of transcriptional and posttranscriptional mechanisms in proximal-distal axis patterning of the Drosophila legs. (C) 1999 Academic Press.