Cloning and molecular analysis of the human citrate synthase gene.
Cloning and molecular analysis of the human citrate synthase gene.
复制标题
人柠檬酸合酶基因的克隆和分子分析。
作者：
M. Goldenthal;J. Marín;R. Ananthakrishnan
通过对从人心脏cDNA文库中分离的柠檬酸合酶（CS）cDNA进行测序，确定了编码CS基因的核苷酸序列。从其核苷酸序列推导的CS的主要序列揭示了一个高度保守的，虽然稍大，466个氨基酸的蛋白质，与其猪同源物的同源性为95%。这些数据还表明，人类基因组CS基因不含内含子，并确认了人类CS基因在12号染色体上的位置。
The nucleotide sequence encoding the citrate synthase (CS) gene was determined from the sequencing of the CS cDNA isolated from a human heart cDNA library. The primary sequence of CS deduced from its nucleotide sequence reveals a highly conserved, albeit slightly larger, protein of 466 amino acids, with 95% homology to its pig homologue. The data also indicate that the human genomic CS gene contains no introns, and confirms the location of the human CS gene on chromosome 12.