Humic acids alleviate the toxicity of reduced graphene oxide modified by nanosized palladium in microalgae
Humic acids alleviate the toxicity of reduced graphene oxide modified by nanosized palladium in microalgae
复制标题
腐殖酸减轻微藻中纳米钯修饰的还原氧化石墨烯的毒性
DOI：
10.1016/j.ecoenv.2022.113794
复制
发表时间：
2022
影响因子：
6.8
通讯作者：
Xiangang Hu
中科院分区：
文献类型：
--
作者：
Xiaokang Li;Yan Yan;Xiaoqiang Li;Li Mu（通讯作者）;Jingqi Zhao;Mingqi Yao;Xiangang Hu
纳米钯修饰的石墨烯家族材料（Pd/GFM）的使用在各个领域迅速加强;然而，环境因素（例如，天然有机物（NOM））对Pd/GFM的转化和生态毒性的影响在很大程度上仍然未知。在本研究中，还原氧化石墨烯改性纳米钯（Pd/rGO）与腐殖酸（HA）在光照下孵育56 d，以探讨NOM的物理化学转化（如缺陷，表面电荷和分散性）和生物毒性（如生长抑制，氧化应激和超微结构损伤对藻类细胞）的Pd/GFM的影响。结果表明，HA增加了Pd/rGO的缺陷和分散性。Pd/rGO诱导微藻细胞生长抑制、细胞超微结构损伤和氧化应激，并激活相应的防御反应（如超氧化物歧化酶、过氧化物酶和谷胱甘肽）。HA通过调节GSH代谢和丙氨酸生物合成途径减弱了Pd/rGO诱导的微藻防御反应。在HA的存在下，由暴露于Pd/rGO引起的细胞壁损伤（即，孔形成）得到恢复，并且原生质体面积减少了28.6%。此外，1.0mg/L的MoS 2纳米粒可使细胞生长抑制、脂质过氧化、线粒体膜电位降低和活性氧生成分别降低1.4- 65.6%、13.9- 26.1%、21.8- 58.3%和9.6- 16.1%。这些研究结果强调，需要考虑HA对Pd/GFM的环境转化和生物毒性的影响，这对Pd/GFM的管理具有重要意义。· HA增加了Pd/rGO的缺陷和分散性。· HA减轻了C.暴露于Pd/rGO的普通人。· HA降低了C.暴露于Pd/rGO的普通人。·上调氨基酸代谢揭示抗氧化防御机制。
The use of graphene-family materials modified by nanosized palladium (Pd/GFMs) has intensified rapidly in various fields; however, the effects of environmental factors ( e.g. , natural organic matter (NOM)) on the transformation and ecotoxicity of Pd/GFMs remain largely unknown. In this study, reduced graphene oxide modified by nanosized Pd (Pd/rGO) was incubated with humic acid (HA) under light irradiation for 56 d to explore the effects of NOM on the physicochemical transformations ( e.g. , defects, surface charges and dispersity) and biological toxicity ( e.g. , growth inhibition, oxidative stress and ultrastructural damage on algae cells) of Pd/GFMs. The results revealed that HA increased the defects and dispersity of Pd/rGO. Growth inhibition, damage to cellular ultrastructures, and oxidative stress in microalgae cells were induced by Pd/rGO, and corresponding defense responses ( e.g. , superoxide dismutase, peroxidase and glutathione) were activated. HA diminished the above defense responses in microalgae triggered by Pd/rGO by regulating GSH metabolism and the alanine biosynthesis pathway. In the presence of HA, cell wall damage ( i.e. , hole formation) caused by exposure to Pd/rGO was restored, and the plasmolysis area was reduced by 28.6 %. In addition, growth inhibition, lipid peroxidation, loss of mitochondrial membrane potential and ROS formation induced by 1.0 mg/L MoS 2 NPs were decreased by 1.4–65.6 %, 13.9–26.1 %, 21.8–58.3 % and 9.6–16.1 %, respectively. These findings highlight the need to consider the effects of HA on the environmental transformation and biological toxicity of Pd/GFMs, which presents significant implications for the management of Pd/GFMs. • HA increases the defects and dispersity of Pd/rGO. • HA mitigates the oxidative stress of C. vulgaris exposed to Pd/rGO. • HA diminishes the defense responses in C. vulgaris exposed to Pd/rGO. • Up-regulated amino acid metabolism reveals antioxidant defense mechanisms.