New insights into an RNAi approach for plant defence against piercing-sucking and stem-borer insect pests
New insights into an RNAi approach for plant defence against piercing-sucking and stem-borer insect pests
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对植物防御刺吸式和螟虫害虫的 RNAi 方法的新见解
DOI：
10.1111/pce.12546
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发表时间：
2015-11-01
影响因子：
7.3
通讯作者：
Miao, Xuexia
中科院分区：
文献类型：
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作者：
Li, Haichao;Guan, Ruobing;Miao, Xuexia
在转基因作物中表达昆虫双链(DS)RNA可以提高植物对生物胁迫的抗性；然而，创造出能够抵御所有害虫的转基因作物是不切实际的。拟南芥Mob1a是器官生长和繁殖所必需的。用dsMob1a浸根后，根的长度和根数明显受到抑制，植株不能抽穗或开花。用荧光标记的dsEYFP(增强型黄色荧光蛋白)浸泡水稻根24小时后，在水稻鞘、茎和取食水稻的飞虱体内观察到荧光。DsEYFP诱导水稻和飞虱体内AGO和DICER的表达水平。当水稻根被dsActin浸泡时，其生长也受到显著抑制。当飞虱或亚洲玉米螟以用含有昆虫目标基因dsRNA的溶液灌溉的水稻或玉米为食时，昆虫的死亡率显著增加。我们的结果表明，dsRNAs可以被作物的根吸收，触发植物和昆虫的RNAi，并提高刺吸虫和二化螟的死亡率。我们还证实了dsRNA在室外条件下是稳定的。这些结果表明，在作物灌溉过程中，根dsRNA浸泡可以作为一种生物杀虫策略。
Insect double-stranded (ds)RNA expression in transgenic crops can increase plant resistance to biotic stress; however, creating transgenic crops to defend against every insect pest is impractical. ArabidopsisMob1A is required for organ growth and reproduction. When Arabidopsis roots were soaked in dsMob1A, the root lengths and numbers were significantly suppressed and plants could not bolt or flower. Twenty-four hours after rice roots were immersed in fluorescent-labelled dsEYFP (enhanced yellow fluorescent protein), fluorescence was observed in the rice sheath and stem and in planthoppers feeding on the rice. The expression levels of Ago and Dicer in rice and planthoppers were induced by dsEYFP. When rice roots were soaked in dsActin, their growth was also significantly suppressed. When planthoppers or Asian corn borers fed on rice or maize that had been irrigated with a solution containing the dsRNA of an insect target gene, the insect's mortality rate increased significantly. Our results demonstrate that dsRNAs can be absorbed by crop roots, trigger plant and insect RNAi and enhance piercing-sucking and stem-borer insect mortality rates. We also confirmed that dsRNA was stable under outdoor conditions. These results indicate that the root dsRNA soaking can be used as a bioinsecticide strategy during crop irrigation.