Molecular differentiation and phylogeny of entomopathogenic nematodes (rhabditida: heterorhabditidae) based on ND4 gene sequences of Mitochondrial DNA.
Molecular differentiation and phylogeny of entomopathogenic nematodes (rhabditida: heterorhabditidae) based on ND4 gene sequences of Mitochondrial DNA.
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基于线粒体 DNA ND4 基因序列的昆虫病原线虫（棒状杆菌科：异杆菌科）的分子分化和系统发育。
DOI：
10.2307/3285747
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
M. Blouin
中科院分区：
文献类型：
--
作者：
J. Liu;R. Berry;M. Blouin
采用聚合酶链反应（PCR）和PCR产物直接测序的方法，对来自世界不同地区的5种15株异虫线虫的线粒体DNA ND4基因进行了部分序列测定。比对的核苷酸和氨基酸序列通过比较序列差异来区分线虫种类，并通过最大简约和似然方法来推断线虫的系统发育。系统发育树的鲁棒性通过bootstrap测试得到验证。根据DNA序列可将15株线虫分离株分为7个单倍型。在更大的尺度上，序列分化显示出4个不同的类群，对应于4个已描述的物种。5株噬菌体异habditis、马氏异habditis mararelatus和hepialius异habditis之间均未发现序列差异。我们的序列数据在使用不同的树构建方法时产生具有相同拓扑结构的系统发育树。大多数关系也通过氨基酸序列在最大简约分析中得到证实。我们对Heterorhabditis物种的分子系统发育支持现有的分类，该分类主要基于形态学和ND4基因的序列差异，允许物种鉴定。
We determined partial ND4 gene sequences of mitochondrial DNA from 15 heterorhabditid nematode isolates, representing 5 species collected from different regions of the world, by using polymerase chain reaction (PCR) and direct-sequencing of PCR products. Aligned nucleotide as well as amino acid sequences were used to differentiate nematode species by comparing sequence divergence and to infer phylogeny of the nematodes by using maximum parsimony and likelihood methods. Robustness of our phylogenetic trees was checked by bootstrap tests. The 15 nematode isolates can be divided into 7 haplotypes based on DNA sequences. On a larger scale, the sequence divergence revealed 4 distinct groups corresponding to 4 described species. No sequence divergence was detected from 5 isolates of Heterorhabditis bacteriophora or between Heterorhabditis marelatus to Heterorhabditis hepialius. Our sequence data yielded phylogenetic trees with identical topologies when different tree-building methods were used. Most relationships were also confirmed by using amino acid sequences in maximum parsimony analysis. Our molecular phylogeny of Heterorhabditis species support an existing taxonomy that is based largely on morphology and the sequence divergence of the ND4 gene permits species identification.