REPEATING STRUCTURE OF CYTOPLASMIC POLY(A)-RIBONUCLEOPROTEIN

REPEATING STRUCTURE OF CYTOPLASMIC POLY(A)-RIBONUCLEOPROTEIN
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DOI：
10.1073/pnas.77.4.1890
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发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
通讯作者：
KORNBERG, RD
KORNBERG, RD
中科院分区：
其他
文献类型：
--
作者：
BAER, BW;KORNBERG, RD

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[小鼠Friend红白血病细胞]细胞质多聚（A）-核糖核蛋白的重复结构通过T2 RNA酶消化显示。获得约27个残基的倍数的片段模式。重复结构容易从纯化的poly（A）和细胞质因子重建。重组对poly（A）是特异性的，如缺乏poly（G）、poly（C）、poly（dA）和tRNA的竞争所示。重复结构在细胞核中不存在，并且显然在运输到细胞质时形成。
A repeating structure of [mouse Friend erythroleukemic cells] cytoplasmic poly(A)-ribonucleoprotein is revealed by digestion with T2 RNase. A pattern of fragments that are multiples of about 27 residues is obtained. The repeating structure is readily reconstituted from purified poly(A) and cytoplasmic factors. Reconstitution is specific for poly(A), as shown by the lack of competition by poly(G), poly(C), poly(dA) and tRNA. The repeating structure is absent from the nucleus, and is apparently formed upon transport to the cytoplasm.