Effects of Benzoapyrene on migration and invasion of lung cancer cells functioning by TNF-α
Effects of Benzoapyrene on migration and invasion of lung cancer cells functioning by TNF-α
复制标题
苯并芘对TNF-α作用的肺癌细胞迁移和侵袭的影响
DOI：
10.1002/jcb.26683
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Yongchun Zhou
中科院分区：
文献类型：
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作者：
Guangqiang Zhao;Zhengxi Wang;Yunchao Huang;Lianhua Ye;Kaiyun Yang;Qiubo Huang;Xiaobo Chen;Guangjian Li;Ying Chen;Jinhui Wang;Yongchun Zhou
本研究旨在探讨苯并芘与肺癌细胞转移的相关机制。我们还做了实验来证明BaP与其潜在靶点TNF-α之间的联系。采用结晶紫法测定两种细胞的半数致死量（IC_（50））。采用真实的实时定量逆转录聚合酶链反应（qRT-PCR）和Western blot检测TNF-α的表达。创伤愈合实验和transwell实验检测BaP和TNF-α对肺癌细胞转移的影响。细胞死亡率随BaP浓度的增加而增加。BaP可增加肺癌转移细胞数。过表达BaP可增强TNF-α通路相关蛋白（TNF-α、NF-κ B [P65]、Caspase 3和Caspase 8）的表达，TNF-αshRNA可抑制BaP对肺癌细胞迁移和侵袭的促进作用。我们的研究验证了BaP对肺癌细胞转移的积极作用。我们还揭示了TNF-α在帮助BaP诱导的肺癌细胞发展中的工具作用。
In this study, we attempted to find out the underlying mechanism of Benzoapyrene and metastasis of lung cancer cells. We also did experiments to testify the connection between BaP and its potential target,TNF‐α. Cell median lethal dose (IC50) of both cells was measured by crystal violet method. Quantitative real‐time reverse transcription PCR (qRT‐PCR) and Western blot were employed to detect the expression ofTNF‐α. Wound healing assay and transwell assay were utilized to testify the impacts of BaP andTNF‐αon the metastasis of lung cancer cells. Cell death rate was elevated with the increase of BaP concentration. BaP increased the number of metastatic cells of lung cancer. The expressions ofTNF‐αpathway‐associated protein (TNF‐α, NF‐kB [P65], Caspase3, and Caspase8) were enhanced by overexpressed BaP.TNF‐αshRNA suppressed the positive effects of BaP on migration and invasion of lung cancer cells. Our study validated the positive effects of BaP on the metastasis of lung cancer cells. We also revealed the instrumental role ofTNF‐αin helping the development of lung cancer cells induced by BaP.