Antioxidant activity of cleomiscosins A and C isolated from Acer okamotoanum
Antioxidant activity of cleomiscosins A and C isolated from Acer okamotoanum
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DOI：
10.1007/bf02977606
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发表时间：
2007-03-01
影响因子：
6.7
通讯作者：
Bae, KiHwan
中科院分区：
文献类型：
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作者：
Jin, WenYi;Thuong, Phuong Thien;Bae, KiHwan
从冈本枫叶和枝条中分离得到两种香豆素木脂素类化合物，分别为考米菌素A(1)和考米菌素C(2)。在此，我们发现2对催化铜离子(Cu2+)或偶氮化合物2，2‘-azobis-(2-amidinopropane)dihydro-chloride(AAPH)产生的自由基所介导的低密度脂蛋白氧化具有密切的抑制作用，其IC(50)S分别为29.5mU和11.9mM。通过电泳分析，我们还观察到2保护载脂蛋白B-100(apoB-100)免受Cu2+诱导的片段化(在5mM时抑制65.3%)。此外，荧光分析清楚地表明，1和2都能保护载脂蛋白B-100免受Cu2+或HOCl2的氧化修饰(1，IC(50)S分别为13.4和8.1mU；2，IC50分别为23.6mU和3.9mU)。这些发现表明，1和2可能有益于防止动脉粥样硬化病变中的低密度脂蛋白氧化。
Phytochemical investigation of Acer okamotoanum leaf and twig led to the isolation of two coumarinolignans, cleomiscosin A (1) and cleomiscosin C (2). Here, we found that 2 close-dependently inhibits LDL oxidation mediated by either catalytic copper ions (Cu2+) or free radicals generated with the azo compound 2,2'-azobis-(2-amidinopropane)dihydro-chloride (AAPH) with IC(50)s of 29.5 and 11.9 mu M, respectively. By electrophoretic analysis, we also observed that 2 protects apolipoprotein B-100 (apoB-100) against Cu2+-induced fragmentation (65.3% inhibition at 5 mu M). Furthermore, fluorescence analyses clearly indicated that both 1 and 2 protect against the oxidative modification of apoB-100 induced by either Cu2+ or HOCl (1, IC(50)s of 13.4 and 8.1 mu M, respectively; 2, IC50s of 23.6 and 3.9 mu M, respectively). These findings suggest that 1 and 2 could be beneficial in preventing LDL oxidation in atherosclerotic lesions.