A group of nuclear factor Y transcription factors are sub-functionalized during endosperm development in monocots
A group of nuclear factor Y transcription factors are sub-functionalized during endosperm development in monocots
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一组核因子 Y 转录因子在单子叶植物胚乳发育过程中被亚功能化
DOI：
10.1093/jxb/ery087
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发表时间：
2018-04-27
影响因子：
6.9
通讯作者：
Chen, Chen
中科院分区：
文献类型：
--
作者：
E, Zhiguo;Li, Tingting;Chen, Chen
核因子Y（NF-Y）是一种异源三聚体转录因子，由NF-YA、NF-YB和NF-YC三个亚基组成。NF-Y在胚乳发育过程中的基因功能尚不清楚。在本研究中，我们鉴定了8个水稻NF-Y编码基因，即OsNF-YA 8、OsNF-YB 1，9和OsNF-YC 8，9，10，11，12，它们主要在胚乳中表达。有趣的是，这些OsNF-Y的同源物仅存在于单子叶植物中，并且也优先在胚乳中表达，这表明它们在胚乳发育的调控中起作用。系统分析水稻胚乳优先NF-Ys在酵母中的相互作用，发现OsNF-YBs和OsNF-YCs可以相互作用。我们还发现，胚乳偏好的OsNFYBs和OsNF-YC可以与水稻的一些乙烯反应因子（ERF）相互作用。与0 sNF-YC 8、9、10不同，0 sNF-YB 1、9或0 sNF-YC 11、12的成员在单独存在时不显示转录激活。然而，它们在二聚体形式时显示出功能活性。此外，OsNF-YB 1基因敲除株系在种子形态上表现出显著变化，进一步证实了其在胚乳发育中的作用。我们的研究结果提供了证据，一组遗传上保守的NF-γ可能是在单子叶植物中分化，以调节胚乳发育。
Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor that consists of three subunits, NF-YA, NF-YB, and NF-YC. Gene functions of NF-Ys during endosperm development are not well understood. In this study, we identified eight rice NF-Y-encoding genes, namely OsNF-YA8, OsNF-YB1,9, and OsNF-YC8,9,10,11,12, that are predominantly expressed in the endosperm. Interestingly, the close homologs of these OsNF-Ys are present only in monocot species and are also preferentially expressed in the endosperm, suggesting that they have roles in the regulation of endosperm development. A systemic analysis of interactions between rice endosperm-preferential NF-Ys in yeast revealed that OsNF-YBs and OsNF-YCs could interact with each other. We also found that the endosperm-preferential OsNFYBs and OsNF-YCs could interact with some ethylene response factors (ERFs) of rice. Unlike OsNF-YC8,9,10, the members of OsNF-YB1,9 or OsNF-YC 11,12 showed no transcriptional activation when present alone. However, they displayed functional activity while in dimer form. In addition, OsNF-YB1-knockout lines showed significant changes in seed morphology, further confirming its role in endosperm development. Our findings provide evidence that a group of phylogenetically conserved NF-Ys is probably differentiated in monocots to regulate endosperm development.