High-frequency 1H NMR studies of the effects of growth factors and phorbol esters on normal Syrian hamster diploid fibroblast cells.
High-frequency 1H NMR studies of the effects of growth factors and phorbol esters on normal Syrian hamster diploid fibroblast cells.
复制标题
高频 1H NMR 研究生长因子和佛波酯对正常叙利亚仓鼠二倍体成纤维细胞的影响。
DOI：
10.1007/bf02797271
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Kan,LS
中科院分区：
文献类型：
--
作者：
Boal,JH;Deamond,SF;Callahan,DE;Bruce,SA;Ts'o,PO;Kan,LS
The nuclear magnetic resonance (NMR) parameters, spin-lattice (T1), and spin-spin (T2) relaxation time, are usually longer for neoplastic cells than for normal cells of the same cell type. This has generally been true at low NMR frequencies (≤100 MHz) when comparisons have been made between normal and neoplastic cells that have both spent a short time in culture. We have previously demonstrated that although the T1values of paired normal and neoplastic Syrian hamster (SH) fibroblastic cells in culture are not significantly different when measured at 300 MHz, the 300 MHz T2values for the neoplastic cells are smaller than those of the normal cells. (Xin et al. (1986),Cell Biophysics8, 213.) Since treatment of normal diploid cells with polypeptide growth factors or tumor promoters frequently results in reversible expression of neoplasia-associated phenotypes, T1and T2were obtained at 300 MHz for treated and untreated SH cells to see if these compounds could also produce smaller 300 MHz T2values. Secondary culture SH fetal fibroblast cells were treated with epidermal growth factor (EGF), fibroblast growth factor (FGF), phorbol-12,13-didecanoate (PDD) and 4-α-phorbol-12,13-didecanoate (4αPDD). Treatment with either growth factor resulted in smaller T2values, but a statistically significant decrease was not observed for PDD or 4αPDD. The observed reductions in T2values were correlated with the morphological and growth-stimulatory effects of these compounds on the cells.
