EMX AND OTX HOMEOBOX GENES IN THE DEVELOPING MOUSE-BRAIN
EMX AND OTX HOMEOBOX GENES IN THE DEVELOPING MOUSE-BRAIN
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DOI：
10.1002/neu.480241008
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发表时间：
1993-10-01
期刊：
影响因子：
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通讯作者：
BROCCOLI, V
中科院分区：
文献类型：
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作者：
BONCINELLI, E;GULISANO, M;BROCCOLI, V
我们分析了四个小鼠同源盒基因的表达，这些基因与果蝇在发育中的头部表达的两个基因有关。其中两个基因，Emx1和Emx2，与空气门有关，两个基因，称为Otx1和Otx2，与正畸有关。这些基因均在E10小鼠胚胎发育的吻侧脑中表达，并且它们的表达域可以比较。Otx2在端脑、间脑和中脑的所有背侧和大部分腹侧区域表达。Otx1的表达域与Otx2的表达域相似，但更小且包含在其中。Emx2表达域包括背端脑和小的间脑区域，包括背侧和腹侧。最后，Emx1的表达仅局限于背端脑。当脑主要区域发生区域规范时，四个基因的表达域呈现出Emx1 < Emx2 < Otx1 < Otx2序列中相互包含的连续区域。四个基因转录本的首次出现也是顺序的：Otx2首先表达（E5.5），其次是Otx1和Emx2 (E8-8.5)，最后是Emx1 （E9.5）。在以背端脑为中心的离散渐进过程中，这四种基因在建立和/或指示各种胚胎脑区域界限方面的可能作用是很诱人的。1993 John Wiley & Sons, Inc.；
We have analyzed the expression of four mouse homeobox genes related to two Drosophila genes expressed in the developing head of the fly. Two of these genes, Emx1 and Emx2, are related to empty spiracles, and two genes, termed Otx1 and Otx2, are related to orthodenticle. These genes are all expressed in the developing rostral brain of E10 mouse embryos and their expression domains can be compared. Otx2 is expressed in all dorsal and most ventral regions of telencephalon, diencephalon, and mesencephalon. The Otx1 expression domain is similar to that of Otx2, but smaller and contained within it. The Emx2 expression domain is comprised of dorsal telencephalon and small diencephalic regions, both dorsally and ventrally. Finally, Emx1 expression is exclusively confined to the dorsal telencephalon. At the time when regional specification of major brain regions takes place, the expression domains of the four genes appear to be continuous regions contained within each other in the sequence Emx1 < Emx2 < Otx1 < Otx2. The first appearance of transcripts of the four genes is also sequential: Otx2 is expressed first (E5.5), followed by Otx1 and Emx2 (E8-8.5), and finally by Emx1 (E9.5). It is tempting to speculate about a possible role of the four genes in establishing and/or signalling the limits of the various embryonic brain regions in a discrete progressive process with its center in the dorsal telencephalon. (C) 1993 John Wiley & Sons, Inc.