Immunological role of thiol-dependent peroxiredoxin gene in Macrobrachium rosenbergii
Immunological role of thiol-dependent peroxiredoxin gene in Macrobrachium rosenbergii
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DOI：
10.1016/j.fsi.2012.04.010
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发表时间：
2012-07-01
影响因子：
4.7
通讯作者：
Bhassu, Subha
中科院分区：
文献类型：
--
作者：
Arockiaraj, Jesu;Easwvaran, Sarasvathi;Bhassu, Subha
在这项研究中，我们报告了一个全长peroxiredoxin（指定MrPrdx）基因，从转录组的淡水虾罗氏沼虾。MrPrdx基因全长940个碱基，编码186个氨基酸。MrPrdx在34和186之间的氨基酸序列中含有长硫氧还蛋白结构域。研究了MrPrdx基因在健康人和感染性皮下和造血组织坏死病毒（IHHNV）的M.采用定量真实的时间聚合酶链反应检测Rosenbertine。MrPrdx在M.被认为是分析的rosenbertus和最高的鳃。IHHNV感染后，在鳃中的表达强烈上调。为了解MrPrdx的功能特性，在大肠杆菌BL21（DE3）中表达并纯化了重组MrPrdx蛋白。使用不同浓度的重组MrPrdx蛋白进行过氧化物酶活性测定。该过氧化物酶活性表明重组MrPrdx是巯基依赖性蛋白。此外，该结果表明重组MrPrdx蛋白作为分泌蛋白，具有清除H2O2和保护DNA损伤的作用。这一发现为提出重组MrPrdx蛋白作为活性氧（ROS）相关疾病的有效药物提供了一种可能的方法。(C)2012爱思唯尔有限公司保留所有权利。
In this study, we have reported a full length of peroxiredoxin (designated MrPrdx) gene, identified from the transcriptome of freshwater prawn Macrobrachium rosenbergii. The complete gene sequence of the MrPrdx is 940 base pairs in length, and encodes 186 amino acids. MrPrdx contains a long thioredoxin domain in the amino acid sequence between 34 and 186. The gene expressions of MrPrdx in healthy and the infectious hypodermal and hematopoietic necrosis virus (IHHNV) challenged M. rosenbergii were examined using quantitative real time polymerase chain reaction. MrPrdx is highly expressed in all the other tissues of M. rosenbergii considered for analysis and the highest in gills. The expression is strongly up-regulated in gills after IHHNV infection. To understand MrPrdx functional properties, the recombinant MrPrdx protein was expressed in Escherichia coli BL21 (DE3) and purified. A peroxidise activity assay was conducted using recombinant MrPrdx protein at different concentrations. This peroxidises activity showed that the recombinant MrPrdx is a thiol-dependant protein. Additionally, this result showed that recombinant MrPrdx protein, as a secretory protein can remove H2O2 and protect DNA damage. This finding leads a possible way to propose the recombinant MrPrdx protein as an effective medicine for reactive oxygen species (ROS) related diseases. (C) 2012 Elsevier Ltd. All rights reserved.