The prevalence rate of periodontal pathogens and its association with oral squamous cell carcinoma
The prevalence rate of periodontal pathogens and its association with oral squamous cell carcinoma
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牙周病原菌的患病率及其与口腔鳞状细胞癌的关系
DOI：
10.1007/s00253-018-9475-6
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发表时间：
2019-02-01
影响因子：
5
通讯作者：
Pan, Yaping
中科院分区：
文献类型：
--
作者：
Chang, Chunrong;Geng, Fengxue;Pan, Yaping
越来越多的证据表明，特定的细菌感染或微生物组成与某些恶性肿瘤的发生发展之间存在因果关系。在本研究中，我们通过16S rRNA扩增子测序、定量聚合酶链反应（qPCR）和荧光原位杂交技术进行研究，以证实牙周病原体与口腔鳞状细胞癌（OSCC）之间的关系。选取6例OSCC患者的龈下菌斑、癌组织及癌旁组织，通过16S rRNA扩增子测序绘制细菌图谱。研究表明，牙周病原体在癌组织和癌旁组织中富集，且癌组织与龈下菌斑的细菌图谱相似。此外，通过qPCR检测了61份癌组织、癌旁组织和龈下菌斑样本以及30份正常组织中牙龈卟啉单胞菌、具核梭杆菌和血链球菌的相对丰度。结果显示，牙龈卟啉单胞菌和具核梭杆菌在癌组织中的含量高于正常组织，且与龈下菌斑相关。使用特殊的寡核苷酸探针检测发现，60.7%的OSCC组织、32.8%的癌旁组织和13.3%的正常组织中存在牙龈卟啉单胞菌。相关性分析表明，牙龈卟啉单胞菌感染与OSCC患者的临床晚期分期、低分化及淋巴结转移呈正相关，同时伴有更深的牙周袋、严重的临床附着丧失和牙齿缺失。本研究揭示了口腔微生物，尤其是牙周病原体，与OSCC之间可能存在密切关系，这或许能丰富口腔鳞癌的发病机制。
Mounting evidence suggests a causal relationship between specific bacterial infections or microbial compositions and the development of certain malignant neoplasms. In this study, we performed research through 16S rRNA amplicon sequencing, qPCR and fluorescence in situ hybridization to certify the relationship between periodontal pathogens and oral squamous cell carcinoma (OSCC). Subgingival plaque, cancer and paracancerous tissues from 6 patients with OSCC were selected for mapping bacterial profiles by 16S rRNA amplicon sequencing. The research showed that periodontal pathogens were enriched in cancer and paracancerous tissues, while the bacterial profiles were similar between the cancer tissues and subgingival plaque. Furthermore, the relative abundance of Porphyromonas gingivalis, Fusobacterium nucleatum and Streptococcus sanguinis was detected in 61 cancer tissues, paracancerous tissues and subgingival plaque samples and in 30 normal tissues by qPCR. The results revealed that P. gingivalis and F. nucleatum existed at higher levels in cancer tissue than in normal tissues and were correlated with subgingival plaques. P. gingivalis was detected using a special oligonucleotide probe in 60.7% of OSCC tissues, 32.8% of paracancerous tissues and 13.3% of normal tissues. Relevance analysis showed that P. gingivalis infection was positively associated with late clinical staging, low differentiation and lymph node metastasis in patients with OSCC, which was accompanied by deeper periodontal pockets, severe clinical attachment loss and loss of teeth. This study revealed that there might be a close relationship between oral microorganisms, particularly periodontal pathogens, and OSCC, which might enrich the pathogenesis of oral squamous carcinoma.