Transmission electron microscopy of tissue culture preparations on polystyrene coverslips.
Transmission electron microscopy of tissue culture preparations on polystyrene coverslips.
复制标题
聚苯乙烯盖玻片上组织培养制剂的透射电子显微镜。
作者:
A. Richters;P. L. Valentin
Polystyrene coverslips have been utilized in preparing tissue cultures for transmission electron microscopy. A method is described for processing the coverslips, including the marking of selected groups of cells with epoxy-carbon mixture. The epoxy-carbon markings aid in locating the cells during sectioning and the method can be used for small or large numbers of cells. In addition, the methodology allows high power phase contrast photography before and after fixation.