ASCORBIC-ACID PHOSPHATE ESTER AND WOUND-HEALING IN RABBIT CORNEAL ALKALI BURNS - EPITHELIAL BASEMENT-MEMBRANE AND STROMA
ASCORBIC-ACID PHOSPHATE ESTER AND WOUND-HEALING IN RABBIT CORNEAL ALKALI BURNS - EPITHELIAL BASEMENT-MEMBRANE AND STROMA
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DOI：
10.1007/bf00918845
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发表时间：
1993-04-01
影响因子：
2.7
通讯作者：
HIKITA, M
中科院分区：
文献类型：
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作者：
SAIKA, S;UENOYAMA, K;HIKITA, M
我们研究了L-抗坏血酸2-磷酸盐（P-Asc）对兔角膜碱烧伤愈合的影响。将含有1 N NaOH的圆形滤纸应用于中央角膜60或120秒以产生碱烧伤。用局部盐水、10%抗坏血酸盐或6.5% P-Asc处理动物的角膜。然后对角膜进行组织学检查。烧伤的基质没有甲苯胺蓝染色，表明糖胺聚糖的损失。在60秒烧伤组，P-Asc减少的未染色区域的大小与对照组相比。透射电子显微镜显示，在抗坏血酸或P-Asc处理的角膜中，新上皮下的基底层，但在对照组中没有。这些观察结果支持了P-Asc可能在角膜碱烧伤修复中具有治疗作用的理论。
We examined the effect of L-ascorbic acid 2-phosphate (P-Asc) on the healing of alkali-burned corneas in rabbits. Round filter paper containing 1 N NaOH was applied to the central cornea for 60 or 120 s to produce the alkali burn. Animals were treated with topical saline, 10% ascorbate, or 6.5% P-Asc applied on the cornea. The corneas were then examined histologically. Burned stroma showed no toluidine blue staining, indicating a loss of glycosaminoglycan. In the 60-s burn group, P-Asc reduced the size of the unstained area as compared with the control. Transmission electron microscopy showed basal lamina under new epithelia in the corneas treated with ascorbate or P-Asc, but not in controls. These observations support the theory that P-Asc may have a therapeutic role in the repair of corneal alkali burns.