Leafy head formation of the progenies of transgenic plants of Chinese cabbage with exogenous auxin genes
Leafy head formation of the progenies of transgenic plants of Chinese cabbage with exogenous auxin genes
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DOI：
10.1038/sj.cr.7290044
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发表时间：
2000-06-01
期刊：
影响因子：
44.1
通讯作者：
Liu, PL
中科院分区：
文献类型：
--
作者：
He, YK;Xue, WX;Liu, PL
进行该实验以评价转源自Ri T-DNA的生长素合成基因的植物系的后代。对4个转基因株系进行自交，经Southern杂交证实T5代植株中含有外源生长素基因。D1232和D1653这两个株系的展开叶折叠时间比未转化株系早，因此叶球的发生也早。转基因株系D1653的叶片插穗比对照产生更多的不定根，而在折叠期折叠叶插穗比莲座叶插穗产生更多的不定根，在抽穗期头叶插穗比莲座叶插穗产生更多的不定根。研究表明，较高浓度的生长素可能是导致转基因植株叶片早期折叠的主要原因。这些转生长素基因植物株系可用于研究植物器官分化发育的激素调控和培育速生新品种。
The experiment was performed to evaluate the progenies of plant lines transgenic for auxin synthesis genes derived from Ri T-DNA. Four lines of the transgenic plants were self-crossed and the foreign auxin genes in plants of T5 generation were confirmed by Southern hybridization. Two lines, D1232 and D1653, showed earlier folding of expanding leaves than untransformed line and therefore had early initiation of leafy head. Leaf cuttings derived from plant of transgenic line D1653 produced more adventitious roots than the control whereas the cuttings from folding leaves had much more roots than rosette leaves at folding stage, and the cuttings from head leaves had more roots than rosette leaves at heading stage. It is demonstrated that early folding of transgenic leaf may be caused by the relatively higher concentration of auxin. These plant lines with auxin transgenes can be used for the study of hormonal regulation in differentiation and development of plant organs and for the breeding of new variety with rapid growth trait.