Microbial Side-Chain Cleavage of Phytosterols by Mycobacteria in Vegetable Oil/Aqueous Two-Phase System
Microbial Side-Chain Cleavage of Phytosterols by Mycobacteria in Vegetable Oil/Aqueous Two-Phase System
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DOI：
10.1007/s12010-014-1082-0
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发表时间：
2014-08
影响因子：
3
通讯作者：
Yangyang Xu;Y. Guan;Hai-Qing Wang;Shanjing Yao
中科院分区：
文献类型：
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作者：
Yangyang Xu;Y. Guan;Hai-Qing Wang;Shanjing Yao
利用分枝杆菌（Mycobacteria）将天然甾醇侧链裂解为4-雄甾烯-3，17-二酮（AD）和1，4-雄甾二烯-3，17-二酮（ADD）的研究已成为制药行业的研究热点，但由于甾醇的疏水性很强，导致该反应产率低，是一个亟待解决的坚韧。八种植物油，即，向日葵、花生、玉米、橄榄、亚麻籽、核桃、葡萄籽和稻米油，用于构建油/水两相体系，用于分枝杆菌MB 3683细胞生物转化植物甾醇。结果表明，植物油适合于植物甾醇的生物转化。特别是在向日葵两相体系（油：水= 1：9）中，在30 °C、200 rpm条件下，以10 g/L的投料浓度进行转化120 h后，AD的产率可达84.8%。测定了AD在不同油/水体系中的分配系数。由于植物油价格低廉且对环境友好，因此油/水两相体系在细菌全细胞生物催化中具有很大的应用潜力。
Microbial side-chain cleavage of natural sterols to 4-androstene-3,17-dione (AD) and 1,4-androstadiene-3,17-dione (ADD) by Mycobacteria has received much attention in pharmaceutical industry, while low yield of the reaction owing to the strong hydrophobicity of sterols is a tough problem to be solved urgently. Eight kinds of vegetable oils, i.e., sunflower, peanut, corn, olive, linseed, walnut, grape seed, and rice oil, were used to construct oil/aqueous biphasic systems in the biotransformation of phytosterols by Mycobacterium sp. MB 3683 cells. The results indicated that vegetable oils are suitable for phytosterol biotransformation. Specially, the yield of AD carried out in sunflower biphasic system (phase ratio of 1:9, oil to aqueous) was greatly increased to 84.8 % with 10 g/L feeding concentration after 120-h transformation at 30 °C and 200 rpm. Distribution coefficients of AD in different oil/aqueous systems were also determined. Because vegetable oils are of low cost and because of their eco-friendly characters, there is a great potential for the application of oil/aqueous two-phase systems in bacteria whole cell biocatalysis.