Dynamics-Function Analysis in Catalytic RNA Using NMR Spin Relaxation and Conformationally Restricted Nucleotides
Dynamics-Function Analysis in Catalytic RNA Using NMR Spin Relaxation and Conformationally Restricted Nucleotides
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使用 NMR 自旋弛豫和构象限制性核苷酸对催化 RNA 进行动力学功能分析
DOI：
10.1007/978-1-0716-0716-9_11
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发表时间：
2021
影响因子：
--
通讯作者：
Sumita, Minako
中科院分区：
文献类型：
--
作者：
Hoogstraten, Charles G.;Terrazas, Montserrat;Aviñó, Anna;White, Neil A.;Sumita, Minako
生物分子功能的充分理解需要分析感兴趣的系统的动态特性和识别功能所需的动态特性。我们描述了基于代谢定向的特定同位素标记的NMR方法，用于识别RNA骨架核糖基团上的分子紊乱和/或构象转变。这些分析的补充，使用合成的共价修饰的核苷酸限制到一个单一的糖皱褶，允许功能评估的动态选择性地删除一个次要的构象异构体确定的NMR从结构系综。
A full understanding of biomolecular function requires an analysis of both the dynamic properties of the system of interest and the identification of those dynamics that are required for function. We describe NMR methods based on metabolically directed specific isotope labeling for the identification of molecular disorder and/or conformational transitions on the RNA backbone ribose groups. These analyses are complemented by the use of synthetic covalently modified nucleotides constrained to a single sugar pucker, which allow functional assessment of dynamics by selectively removing a minor conformer identified by NMR from the structural ensemble.