Comparative studies on the chemical and immunochemical properties of human milk, human pancreatic juice and bovine milk lactoferrin.
Comparative studies on the chemical and immunochemical properties of human milk, human pancreatic juice and bovine milk lactoferrin.
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人乳、人胰液和牛乳乳铁蛋白的化学和免疫化学特性的比较研究。
DOI：
10.1016/0305-0491(84)90100-7
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
H. Kloer
中科院分区：
文献类型：
--
作者：
C. S. Wang;W. Chan;H. Kloer
采用肝素-琼脂糖亲和层析法从人乳、胰液和牛乳中分离纯化乳铁蛋白。这些乳铁蛋白制剂的尿素-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示相似的分子量（80，000）。金属分析表明，牛乳中的乳铁蛋白含有最高量的铁，而人乳和人胰液中的乳铁蛋白的铁含量相似，比牛乳低约四倍。还发现所有这些乳铁蛋白制剂含有少量的铜和锰。双扩散分析表明人乳中的乳铁蛋白与人胰液中的乳铁蛋白在免疫化学上是相同的。另一方面，免疫化学，牛乳乳铁蛋白是不相同的人乳乳铁蛋白。人乳和胰液乳铁蛋白的序列分析表明，它们共享相同的N-末端序列的16个残基分析。虽然人乳和牛乳乳铁蛋白具有序列同源性，但牛乳乳铁蛋白与卵转铁蛋白的同源性比与人乳乳铁蛋白的同源性更接近。
Lactoferrin from human milk, pancreatic juice and bovine milk were purified by heparin-Sepharose affinity chromatography procedure. Urea-sodium dodecylsulfate-polyacrylamide gel electrophoresis of these lactoferrin preparations indicated similar molecular weights (80,000). Metal analyses showed that lactoferrin of bovine milk contained the highest amount of iron while lactoferrin of human milk and human pancreatic juice were similar in content of iron, approximately four-fold lower than bovine milk. All these lactoferrin preparations were also found to contain minor amounts of copper and manganese. Double diffusion analyses indicated that lactoferrin of human milk was immunochemically identical to lactoferrin of human pancreatic juice. On the other hand, immunochemically, bovine milk lactoferrin was not identical to human milk lactoferrin. Sequence analyses of human milk and pancreatic juice lactoferrin indicated that they shared the same N-terminal sequence for the 16 residues analyzed. Although human milk and bovine milk lactoferrin had sequence homologies, bovine milk lactoferrin had a closer homology to ovotransferrin than to human milk lactoferrin.