SIMIAN VIRUS 40 DEOXYRIBONUCLEIC ACID SYNTHESIS - VIRAL REPLICON
SIMIAN VIRUS 40 DEOXYRIBONUCLEIC ACID SYNTHESIS - VIRAL REPLICON
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DOI：
10.1128/jvi.10.4.591-598.1972
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发表时间：
1972-01-01
影响因子：
5.4
通讯作者：
TEGTMEYER, P
中科院分区：
文献类型：
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作者：
TEGTMEYER, P
猴病毒40（SV40）互补组A中的三个温度敏感（ts）突变体（tsA7、tsA28、tsA30）已在允许和限制性宿主细胞中分离和表征。在41 ℃时，非洲绿色猴肾细胞的AH系中，突变体缺乏产生传染性病毒脱氧核糖核酸（DNA）所需的早期功能。通过凝胶电泳对SV40病毒DNA复制的温度变化实验和分析提供了强有力的证据，即三种突变体的ts基因产物直接需要启动每一轮新的病毒DNA复制，但不需要完成已经开始的循环。在41 ℃的病毒互补研究中，突变DNA分子本身的合成可由非突变基因产物引发。然而，细胞不能替代宿主功能来提供游离病毒DNA复制所需的起始物。还需要病毒引发剂来建立3T3细胞的稳定转化。
Three temperature-sensitive (ts) mutants of simian virus 40 (SV40) in complementation group A (tsA7, tsA28, tsA30) have been isolated and characterized in permissive and restrictive host cells. At 41 C in the AH line of African green monkey kidney cells, the mutants are deficient in an early function required to produce infectious viral deoxyribonucleic acid (DNA). Temperature-shift experiments and analysis of SV40 viral DNA replication by gel electrophoresis have provided strong evidence that the ts gene product of the three mutants is directly required to initiate each new round of viral DNA replication but is not required to complete a cycle which has already begun. The synthesis of mutant DNA molecules themselves can be initiated by a nonmutant gene product in viral complementation studies at 41 C. The cell, however, cannot substitute a host function to provide the initiator required for the replication of free viral DNA. The viral initiator is also required to establish the stable transformation of 3T3 cells.