Kinetic and concentration analysis using BIA technology

Kinetic and concentration analysis using BIA technology
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DOI:
10.1006/meth.1994.1013
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发表时间:
1994-01-01
期刊:
Methods (Orlando)
影响因子:
--
通讯作者:
Persson, Bjorn
Persson, Bjorn
中科院分区:
其他
文献类型:
--
作者:
Karlsson, Robert;Roos, Hakan;Persson, Bjorn

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表面等离子体共振检测使直接测量溶液中分析物与表面固定结合伙伴的结合成为可能。一个结合配对的固定化、靠近传感器表面的分析物的可能耗尽以及假定的相互作用模型在结合数据的解释中是重要的。我们描述了固定化的一些可能的影响,低结合能力表面的动力学分析,高结合能力表面的浓度分析,以及如何从结合数据中识别偏离1:1相互作用模型的情况。
Surface plasmon resonance detection makes it possible to directly measure the binding of an analyte in solution to a surface immobilized binding partner. The immobilization of one binding partner, the possible depletion of analyte close to the sensor surface, and the assumed interaction model are important in the interpretation of binding data. We describe some of the possible effects of immobilization, kinetic analysis on surfaces with low binding capacity, concentration analysis on surfaces with high binding capacity, and how deviations from a 1:1 interaction model may be identified from binding data.