Yellow fever 17D-vectored vaccines expressing Lassa virus GP1 and GP2 glycoproteins provide protection against fatal disease in guinea pigs.
Yellow fever 17D-vectored vaccines expressing Lassa virus GP1 and GP2 glycoproteins provide protection against fatal disease in guinea pigs.
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表达拉沙病毒 GP1 和 GP2 糖蛋白的黄热病 17D 载体疫苗可为豚鼠提供针对致命疾病的保护。
DOI：
10.1016/j.vaccine.2010.11.079
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发表时间：
2011-02-01
期刊：
影响因子：
5.5
通讯作者：
Lukashevich, Igor S.
中科院分区：
文献类型：
--
作者：
Jiang, Xiaohong;Dalebout, Tim J.;Bredenbeek, Peter J.;Carrion, Ricardo, Jr.;Brasky, Kathleen;Patterson, Jean;Goicochea, Marco;Bryant, Joseph;Salvato, Maria S.;Lukashevich, Igor S.
黄热病（YF）和拉沙热（LF）是西非共同流行的两种流行出血热，每年造成数千人死亡。YF疫苗17 D已被用作拉沙病毒糖蛋白前体（LASV-GPC）或其亚基GP 1（附着糖蛋白）和GP 2（融合糖蛋白）的载体。在YF 17 D E和NS 1基因之间克隆较短的插入片段LASV GP 1和GP 2，与YF 17 D/LASV GPC重组体相比，提高了重组病毒YF 17 D/LASV GP 1和-GP 2的遗传稳定性。重组病毒具有复制能力，并在感染细胞中正确加工YF和LASV GP蛋白。YF 17 D/LASV-GP 1和GP 2在小鼠中诱导特异性CD 8 + T细胞应答并保护13株豚鼠对抗致死性LF。与用减毒活疫苗ML 29免疫不同，用YF 17 D/LASV-GP 1和GP 2免疫不提供杀菌免疫。本研究证明了基于YF 17 D的疫苗在西非控制LF的可行性。
Yellow Fever (YF) and Lassa Fever (LF) are two prevalent hemorrhagic fevers co-circulating in West Africa and responsible for thousands of deaths annually. The YF vaccine 17D has been used as a vector for the Lassa virus glycoprotein precursor (LASV-GPC) or their subunits, GP1 (attachment glycoprotein) and GP2 (fusion glycoprotein). Cloning shorter inserts, LASV GP1 and GP2, between YF17D E and NS1 genes enhanced genetic stability of recombinant viruses, YF17D/LASV-GP1 and –GP2, in comparison with YF17D/LASV-GPC recombinant. The recombinant viruses were replication competent and properly processed YF and LASV GP proteins in infected cells. YF17D/LASV-GP1&GP2 induced specific CD8+ T cell responses in mice and protected strain 13 guinea pigs against fatal LF. Unlike immunization with live attenuated reassortant vaccine ML29, immunization with YF17D/LASV-GP1&GP2 did not provide sterilizing immunity. This study demonstrates the feasibility of YF17D-based vaccine to control LF in West Africa.
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影响因子：
5.5
作者：
Barban, V.;Girerd, Y.;Lang, J.
通讯作者：
Lang, J.
DOI：
10.1073/pnas.221447598
发表时间：
2001-10-23
影响因子：
11.1
作者：
Lenz, O;ter Meulen, J;Garten, W
通讯作者：
Garten, W
DOI：
10.1084/jem.20051352
发表时间：
2005-11-07
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Barba-Spaeth G;Longman RS;Albert ML;Rice CM
通讯作者：
Rice CM
影响因子：
3.8
作者：
Fichet-Calvet, Elisabeth;Rogers, David John
通讯作者：
Rogers, David John
影响因子：
4.8
作者：
Bonaldo, Myrna C.;Mello, Samanta M.;Galler, Ricardo
通讯作者：
Galler, Ricardo