BIOCHEMICAL-CHARACTERIZATION OF HUMAN ERYTHROCYTES FRACTIONATED BY COUNTERCURRENT DISTRIBUTION IN AQUEOUS POLYMER 2-PHASE SYSTEMS
BIOCHEMICAL-CHARACTERIZATION OF HUMAN ERYTHROCYTES FRACTIONATED BY COUNTERCURRENT DISTRIBUTION IN AQUEOUS POLYMER 2-PHASE SYSTEMS
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DOI：
10.1016/0021-9673(94)80105-3
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发表时间：
1994-05-06
影响因子：
4.1
通讯作者：
LUQUE, J
中科院分区：
文献类型：
--
作者：
PINILLA, M;DELAFUENTE, J;LUQUE, J
在电荷敏感的右旋糖酐-聚乙二醇两相系统中通过逆流分布（CCD）对正常人红细胞进行分级已得到证实，并扩展到杂合性β-地中海贫血患者的红细胞。正常（同质）和异常（异质）红细胞分布曲线之间的差异反映了它们不同的表面电荷特性。正如神经氨酸酶处理后膜唾液酸释放的下降以及分布曲线（从左到右部分）中两种年龄依赖性酶（膜乙酰胆碱酯酶和细胞内丙酮酸激酶）的比活性所表明的，分级似乎是根据红细胞年龄。在老化的红细胞中观察到 2,3-二磷酸甘油酸水平恒定。
The fractionation of normal human erythrocytes by counter-current distribution (CCD) in charge-sensitive dextran-polyethylene glycol two-phase systems was confirmed and extended to red blood cells from heterozygous beta-thalassaemic patients. The differences between the distribution profiles of normal (homogeneous) and abnormal (heterogeneous) red blood cells reflect their different surface-charge properties. As suggested by the decline of membrane sialic acid released after neuraminidase treatment and the specific activities of two age-dependent enzymes (membrane acetylcholinesterase and intracellular pyruvate kinase) in the distribution profiles (from the left- to the right-hand side fractions), the fractionation seems to be according to red blood cell age. A constancy of the 2,3-bisphosphoglycerate level was observed in ageing red blood cells.