Unique and redundant roles for HOG MAPK pathway components as revealed by whole-genome expression analysis
Unique and redundant roles for HOG MAPK pathway components as revealed by whole-genome expression analysis
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DOI：
10.1091/mbc.e03-07-0521
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发表时间：
2004-02-01
影响因子：
3.3
通讯作者：
Herskowitz, I
中科院分区：
文献类型：
--
作者：
O'Rourke, SM;Herskowitz, I
酿酒酵母高渗透压甘油（HOG）丝裂原活化蛋白激酶途径是酿酒酵母适应所必需的，包含两个分支，通过丝裂原活化蛋白激酶激酶（Pbs 2）激活丝裂原活化蛋白激酶（Hog 1）。我们已经通过使用全基因组表达谱来表征共同途径组分（Hog 1和Pbs 2）和两个上游分支（Ste 11，Sho 1和Ssk 1）中的组分对渗透压升高的响应的作用。揭示了HOG通路的几个新特征。首先，Hog 1在基因诱导和抑制，串扰抑制，并在管理的调节期的功能。第二，pbs 2和hog 1突变体的表型是相同的，表明Pbs 2的唯一作用是激活Hog 1。第三，存在的基因，其诱导是依赖于Hog 1和Pbs 2，但不对Ste 11和Ssk 1表明，有额外的输入到Pbs 2在我们的诱导条件下。第四，两个上游途径分支不是多余的：对于通过适度渗透压激活Pbs 2，Sln 1-Ssk 1分支比Sho 1-Ste 11分支具有显著得多的作用。最后，一般的应激反应途径和HOG途径的两个分支都在高渗透压下起作用。这些研究表明，细胞通过在不同条件下使用不同的信号转导机制来响应增加的渗透压。
The Saccharomyces cerevisiae high osmolarity glycerol (HOG) mitogen-activated protein kinase pathway is required for osmoadaptation and contains two branches that activate a mitogen-activated protein kinase (Hog1) via a mitogen-activated protein kinase kinase (Pbs2). We have characterized the roles of common pathway components (Hog1 and Pbs2) and components in the two upstream branches (Ste11, Sho1, and Ssk1) in response to elevated osmolarity by using whole-genome expression profiling. Several new features of the HOG pathway were revealed. First, Hog1 functions during gene induction and repression, cross talk inhibition, and in governing the regulatory period. Second, the phenotypes of pbs2 and hog1 mutants are identical, indicating that the sole role of Pbs2 is to activate Hog1. Third, the existence of genes whose induction is dependent on Hog1 and Pbs2 but not on Ste11 and Ssk1 suggests that there are additional inputs into Pbs2 under our inducing conditions. Fourth, the two upstream pathway branches are not redundant: the Sln1-Ssk1 branch has a much more prominent role than the Sho1-Ste11 branch for activation of Pbs2 by modest osmolarity. Finally, the general stress response pathway and both branches of the HOG pathway all function at high osmolarity. These studies demonstrate that cells respond to increased osmolarity by using different signal transduction machinery under different conditions.