Cloning and biological characterization of buffalo (Bubalus bubalis) interferon-gamma.
Cloning and biological characterization of buffalo (Bubalus bubalis) interferon-gamma.
复制标题
水牛（Bubalus bubalis）干扰素-γ的克隆和生物学特性。
DOI：
10.1016/j.molimm.2005.03.012
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发表时间：
2006
影响因子：
3.6
通讯作者：
T. Rasool
中科院分区：
文献类型：
--
作者：
A. Premraj;E. Sreekumar;T. Rasool
从分子水平对印度水牛(Bubalus Bubalis)的主要免疫调节细胞因子干扰素-γ进行了研究。对互补DNA和必需启动子区域进行克隆和测序，并在细菌系统中表达了功能性重组蛋白。该基因与11个核苷酸和4个氨基酸变异的核苷酸同源性为97.8%，与相应的牛的相应序列相比，基本启动子区与5个核苷酸变异和4个核苷酸缺失的同源性为98.4%。所有的主要启动子元件，如NFIL-2类基序、环孢素敏感结合元件和GATA基序都是严格保守的。在细菌系统中表达的重组水牛干扰素-γ与抗牛干扰素-γ单抗在Western印迹中反应，在培养的马丁达比牛肾细胞中通过抑制病毒诱导的细胞病变而显示出抗水痘病毒的活性。研究表明，反刍动物之间的干扰素-γ序列具有很高的相似性。鉴于干扰素-γ的免疫调节和抗病毒活性，该分子将有助于更好地了解水牛的免疫系统。
Interferon-γ, a major immunomodulatory cytokine, of Indian water buffalo (Bubalus bubalis) was characterized at molecular level. Complementary DNA and essential promoter region were cloned and sequenced, and functional recombinant protein was expressed in bacterial system. The cDNA has 97.8% nucleotide identity with 11-nucleotide and four-amino acid variations, and the essential promoter region has 98.4% identity with five-nucleotide variations and a four-nucleotide deletion in comparison with the corresponding bovine sequences. All the major promoter elements such as NF IL-2 like motif, cyclosporin sensitive binding element and GATA motif are strictly conserved. Recombinant buffalo-IFN-γ expressed in bacterial system reacted with an anti-bovine-IFN-γ monoclonal antibody in Western blot and showed antiviral activity against buffalo pox virus in cultured Madin-Darby bovine kidney (MDBK) cells by inhibiting virus induced cytopathic effect. The study shows high level sequence similarity of IFN-γ among ruminants. In view of the immunomodulatory and antiviral activities of IFN-γ, this molecule will be useful in better understanding of the immune system of water buffaloes.
IFN-γ 的 N 末端和 C 末端是克隆的可溶性 IFN-γ 受体的结合域。
DOI：
--
发表时间：
1992
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Griggs,ND;Jarpe,MA;Pace,JL;Russell,SW;Johnson,HM
通讯作者：
Johnson,HM