Free radical activity and antioxidant defense mechanisms in patients with hyperthyroidism due to Graves' disease during therapy
Free radical activity and antioxidant defense mechanisms in patients with hyperthyroidism due to Graves' disease during therapy
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DOI：
10.1016/s0009-8981(00)00306-5
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发表时间：
2000-10-01
影响因子：
5
通讯作者：
Kotulska, A
中科院分区：
文献类型：
--
作者：
Komosinska-Vassev, K;Olczyk, K;Kotulska, A
自由基介导的氧化应激与几种自身免疫性疾病的发病机制有关。我们研究了促氧化剂-抗氧化剂状态，以评估氧化现象在Graves病患者中可能的有害作用。本文报告30例Graves病甲亢患者。30名年龄匹配的健康受试者作为对照组进行研究。自由基活性指数，抗氧化防御系统，甲状腺和垂体激素水平测定空腹血液样本。在开始治疗前和达到甲状腺功能正常状态后采集血样。脂质过氧化活性指数显著增加，即，在未经治疗的Graves病患者血清中发现了共轭二烯和硫代巴比妥酸反应物质。这些变化伴随着血浆巯基和红细胞裂解物巯基浓度的降低。甲状腺功能亢进导致细胞内抗氧化酶的显着增加。即，超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性。细胞外抗自由基清除系统的潜力，谷胱甘肽还原酶活性和总的抗氧化状态水平，被认为是显着降低未经治疗的Graves'患者。甲巯咪唑治疗导致自由基和抗氧化活性指数正常化。所获得的结果表明，增强产生的活性氧和损害的细胞和细胞外的抗氧化系统的潜力与格雷夫斯病患者。甲状腺功能正常状态的实现导致氧化应激指数和抗氧化潜力参数的改善。(C)2000爱思唯尔科技有限公司。保留所有权利。
Free radical-mediated oxidative stress has been implicated in the etiopathogenesis of several autoimmune disorders. We investigated the prooxidant-antioxidant status in order to evaluate the possible deleterious role of oxidative phenomena in patients with Graves' disease. Thirty patients with hyperthyroidism due to Graves' disease were investigated. Thirty age-matched healthy subjects were studied as a control group. Free radical activity indices, antioxidant defense systems, and thyroid and pituitary hormone levels were measured in fasting blood samples. Blood samples were taken before initiation of therapy and after attainment of euthyroid state. A significant increase in lipid peroxidation activity indices, i.e., conjugated dienes and thiobarbituric acid-reacting substances, was found in blood serum of the patients with untreated Graves' disease. These changes were accompanied by a decrease in plasma thiol and erythrocyte lysate thiol groups concentrations. Hyperthyroidism resulted in a marked increase in intracellular antioxidant enzymes. i.e.. superoxide dismutase, catalase and glutathione peroxidase activities as compared to the controls. Extracellular anti-free radical scavenging systems potential, measured by glutathione reductase activity and total antioxidant status level, was found to be significantly decreased in untreated Graves' patients. Treatment with thiamazole resulted in normalization of the free radical and antioxidant activity indices. The obtained results indicate an enhanced generation of reactive oxygen species and impairment of cellular and extracellular antioxidant systems potential in patients with Graves' disease. The attainment of euthyroid state led to an improvement in oxidative stress indices and antioxidant potential parameters. (C) 2000 Elsevier Science B.V. All rights reserved.