Characterization of neocortical non-pyramidal neurons expressing preprotachykinins A and B: A double immunofluorescence study in the rat
Characterization of neocortical non-pyramidal neurons expressing preprotachykinins A and B: A double immunofluorescence study in the rat
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DOI：
10.1016/s0306-4522(98)00036-0
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发表时间：
1998-10-01
期刊：
影响因子：
3.3
通讯作者：
Mizuno, N
中科院分区：
文献类型：
--
作者：
Kaneko, T;Murashima, M;Mizuno, N
用免疫细胞化学方法观察了大鼠新皮层表达前原速激肽A和前原速激肽B（分别为P物质和神经激肽B（神经介肽K）的前原肽）的神经元。针对前速激肽原的C-末端部分提出的抗体用于标记前速激肽原产生神经元的细胞体。前速激肽原B免疫反应阳性的神经元在新皮层中出现的频率是前速激肽原A免疫反应阳性神经元的4倍。前速激肽原A免疫反应神经元更频繁地分散在皮层深层（第四-第六层）比在表层（第一-第三层），而前速激肽原B免疫反应神经元更频繁地分布在表层比在深层。几乎所有的前速激肽原表达神经元GABA的免疫反应，这表明他们是非锥体细胞。然而，两个前速激肽原的免疫反应性在单个神经元中的共表达没有发现。前速激肽原表达的新皮层神经元进一步检查与GABA能皮层神经元的亚群的标记。小清蛋白，钙结合蛋白和生长抑素的免疫反应，分别发现在69%，27%和11%，前速激肽A-免疫反应阳性神经元。相反，前速激肽原A免疫反应神经元仅占小清蛋白免疫反应神经元的6%，钙结合蛋白免疫反应神经元的4%和生长抑素免疫反应神经元的1%。在13-39%的前速激肽原B免疫反应神经元中检测到钙视黄蛋白、胆碱乙酰转移酶、血管活性肠多肽、促肾上腺皮质激素释放因子和胆囊收缩素的免疫反应。前速激肽B免疫反应性见于33%的钙视黄蛋白阳性神经元、45%的胆碱能神经元、47%的血管活性肠多肽阳性神经元、59%的促肾上腺皮质激素释放因子阳性神经元和83%的胆囊收缩素阳性神经元。由于P物质和神经激肽B的受体在GABA能神经元[Kaneko T. et al.（1994）Neuroscience 60，199-211]和锥体神经元[Ding Y. Q.呃尼。（1996）J. comp. Neurol 364，290-310]，皮质神经元可以使用两条单独的速激肽信号线;物质P用作GABA能非锥体神经元之间的信号，而神经激肽B用作GABA能神经元到锥体神经元的信号。(C)1998年IBRO。出版社：Elsevier Science Ltd
Neurons expressing preprotachykinin A and preprotachykinin B, which prepropeptides of substance P and neurokinin B (neuromedin K), respectively, were characterized immunocytochemically in the rat neocortex. Antibodies raised against C-terminal portions of preprotachykinins were used for labeling cell bodies of preprotachykinin-producing neurons. Neurons immunoreactive for preprotachykinin B were encountered four times more frequently in the neocortex than those immunoreactive for preprotachykinin A. Preprotachykinin A-immunoreactive neurons were scattered more frequently in the deep cortical layers (layers IV-VI) than in the superficial layers (layers I-III), whereas preprotachykinin B-immunoreactive neurons were distributed more frequently in the superficial layers than in the deep layers. Almost all preprotachykinin-expressing neurons were immunoreactive for GABA, suggesting that they were non-pyramidal cells. However, co-expression of the two preprotachykinin immunoreactivities in single neurons was not found.Preprotachykinin-expressing neocortical neurons were further examined with markers for subpopulations of GABAergic cortical neurons. Immunoreactivities for parvalbumin, calbindin and somatostatin were found in 69%, 27% and 11%, respectively, of preprotachykinin A-immunoreactive neurons. Conversely, preprotachykinin A-immunoreactive neurons constituted only 6% of parvalbumin-immunoreactive neurons, 4% of calbindin-immunoreactive neurons and 1% of somatostatin-immunoreactive neurons. Immunoreactivities for calretinin, choline acetyltransferase, vasoactive intestinal polypeptide, corticotropin-releasing factor and cholecystokinin were detected in 13-39% of preprotachykinin B-immunoreactive neurons. Preprotachykinin B immunoreactivity was seen in 33% of calretinin-positive neurons, 45% of cholinergic neurons, 47% of vasoactive intestinal polypeptide-positive neurons, 59% of corticotropin-releasing factor-positive neurons and 83% of cholecystokinin-positive neurons.These results indicate that preprotachykinin A- and preprotachykinin B-expressing neurons constitute separate populations of GABAergic non-pyramidal neurons in the neocortex. Since receptors for substance P and neurokinin B are expressed in GABAergic neurons [Kaneko T.el al. (1994) Neuroscience 60, 199-211] and pyramidal neurons [Ding Y. Q. er ni. (1996) J. comp. Neurol 364, 290-310], respectively, cortical neurons may use two separate lines of tachykinin signals; substance P serves as a signal between GABAergic non-pyramidal neurons, whereas neurokinin B acts as a signal of GABAergic neurons to pyramidal neurons. (C) 1998 IBRO. Published by Elsevier Science Ltd.