Inductive processes leading to inner ear formation during Xenopus development
Inductive processes leading to inner ear formation during Xenopus development
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DOI：
10.1006/dbio.1996.0098
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发表时间：
1996-04-10
影响因子：
2.7
通讯作者：
Grainger, RM
中科院分区：
文献类型：
--
作者：
Gallagher, BC;Henry, JJ;Grainger, RM
这项研究检查了非洲爪蟾胚胎内耳感应的空间和时间属性。将这些结果与最近有关晶状体感应的实验进行比较，以评估头部感觉结构是否具有共同的个体发育特征。来自不同区域和阶段的外胚层被移植到早期（神经板）或晚期（神经管）宿主的假定耳区。还从这些阶段的胚胎中取出假定的耳外胚层的外植体，以确定耳外胚层规范的时间。我们发现外胚层对于耳的能力。囊泡形成贯穿神经板阶段，比晶状体形成的时间长得多。在神经板阶段，耳囊泡的形成比晶状体的形成要早得多。当暴露于神经板阶段或神经管阶段的耳诱导环境时，有能力的外胚层在大部分情况下形成耳囊泡，这一结果与晶状体诱导形成对比，在晶状体诱导中，神经管阶段胚胎提供比早期阶段弱得多的诱导环境。在神经管阶段宿主中诱导的耳囊泡主要与假定的后脑接触，表明该神经组织可能足以诱导耳囊泡。这些研究揭示了晶状体和内耳感应之间的总体相似性，但也存在足够的差异，表明这些感觉组织的某些方面的决定可能是通过独立的机制而不是通过共同的发育状态发生的。 (C) 1996 学术出版社
This study examines the spatial and temporal attributes of inner ear induction in Xenopus embryos. These results are compared to recent experiments concerning lens induction to assess whether head sensory structures share common ontogenetic features. Ectoderm from different regions and stages was transplanted to the presumptive ear region of hosts of either early (neural plate) or late (neural tube) stages. Explants of the presumptive ear ectoderm were also taken from embryos at these stages to establish the time of otic ectoderm specification. We find that ectodermal competence for otic. vesicle formation extends through neural plate stages, far longer than for lens formation. Otic vesicle specification occurs substantially earlier, at neural plate stages, than lens specification. Competent ectoderm forms otic vesicles in a high fraction of cases when exposed to the ear-inducing environment of either neural plate stages or neural tube stages, a result which contrasts with lens induction where the neural tube stage embryo provides a much weaker inducing environment than earlier stages. Otic vesicles induced in neural tube stage hosts are primarily in contact with presumptive hindbrain, suggesting that this neural tissue may be sufficient for otic vesicle induction. These studies reveal overall similarities between lens and inner ear induction, but sufficient differences to propose that some facets of determination of these sensory tissues may occur by independent mechanisms and not via a common developmental state. (C) 1996 Academic Press, Inc.