Hematopoietic stem/progenitor cells, generation of induced pluripotent stem cells, and isolation of endothelial progenitors from 21-to 23.5-year cryopreserved cord blood
Hematopoietic stem/progenitor cells, generation of induced pluripotent stem cells, and isolation of endothelial progenitors from 21-to 23.5-year cryopreserved cord blood
复制标题
DOI：
10.1182/blood-2011-01-330514
复制
发表时间：
2011-05-05
期刊：
影响因子：
20.3
通讯作者：
Yoder, Mervin C.
中科院分区：
文献类型：
--
作者：
Broxmeyer, Hal E.;Lee, Man-Ryul;Yoder, Mervin C.
造血干细胞(HSCs)和造血祖细胞(HPC)的超低温保存是脐带血(CB)储存和移植的关键。我们评估了作为单核细胞或未分离细胞冷冻保存长达23.5年的功能性HPC的恢复率，并与相同CB单位的冻存前的值进行了比较。粒-巨噬细胞和多向造血祖细胞的高效回收率(80%-100%)是明显的，尽管有些采集具有可重复性的低回收率。增殖潜能、对多种细胞因子的反应和HPC集落的复制是广泛的。从冷冻保存长达21年的脐血中分离出的CD34(+)细胞在原发和继发免疫缺陷小鼠中具有长期(=6个月)移植能力，反映了长期再生、自我更新的造血干细胞的恢复。我们回收了功能反应性的CD4(+)和CD8(+)T淋巴细胞，在体外和体内培养出了代表所有三种生殖细胞系的诱导多能干细胞(IPS)，并检测到高增殖的内皮细胞集落形成细胞，结果与CB生物学和Bank相关。(血。2011；117(18)：4773-4777)
Cryopreservation of hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) is crucial for cord blood (CB) banking and transplantation. We evaluated recovery of functional HPC cryopreserved as mononuclear or unseparated cells for up to 23.5 years compared with prefreeze values of the same CB units. Highly efficient recovery (80%-100%) was apparent for granulocyte-macrophage and multipotential hematopoietic progenitors, although some collections had reproducible low recovery. Proliferative potential, response to multiple cytokines, and replating of HPC colonies was extensive. CD34(+) cells isolated from CB cryopreserved for up to 21 years had long-term (>= 6 month) engrafting capability in primary and secondary immunodeficient mice reflecting recovery of long-term repopulating, self-renewing HSCs. We recovered functionally responsive CD4(+) and CD8(+) T lymphocytes, generated induced pluripotent stem (iPS) cells with differentiation representing all 3 germ cell lineages in vitro and in vivo, and detected high proliferative endothelial colony forming cells, results of relevance to CB biology and banking. (Blood. 2011; 117(18): 4773-4777)