Characteristic fragmentation of bacteriohopanepolyols during atmospheric pressure chemical ionisation liquid chromatography/ion trap mass spectrometry.
Characteristic fragmentation of bacteriohopanepolyols during atmospheric pressure chemical ionisation liquid chromatography/ion trap mass spectrometry.
复制标题
大气压化学电离液相色谱/离子阱质谱分析过程中细菌藿烷多元醇的特征断裂。
DOI：
10.1002/rcm.1265
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
P. Farrimond
中科院分区：
文献类型：
--
作者：
H. Talbot;R. Summons;L. Jahnke;P. Farrimond
在大气压化学电离液相色谱/离子阱质谱（APCI-LC/MS（n））过程中，细菌藿烷多元醇（BHP）通过特征途径裂解。比较细菌藿烷-32，33，34，35-四醇（BHT）和2 β-甲基细菌藿烷-32，33，34，35-四醇的MS（2）光谱，证实了先前提出的C-9和11以及C-8和14之间发生的环-C断裂。这种片段化，所有hopanoids的诊断，也发生在BHP中含有一个氨基（-NH（2））在C-35，虽然较高的相对稳定性的离子限制这种片段化后的一个小过程的基本氮功能质子化。一些细胞培养物，包括prochlorophyte（Prochlorothrix hollandica）和蓝藻（Chlorogloeopsis LA）的研究表明，这种技术的力量，以检测复合BHP在C-35的复杂的生物功能。我们还报告了使用这种方法首次观察到的完整的pentefunctionalised bacteriophopane多元醇。
Bacteriohopanepolyols (BHPs) fragment via characteristic pathways during atmospheric pressure chemical ionisation liquid chromatography/ion trap mass spectrometry (APCI-LC/MS(n)). Comparison of the MS(2) spectra of bacteriohopane-32,33,34,35-tetrol (BHT) and 2 beta-methylbacteriohopane-32,33,34,35-tetrol has confirmed the previously proposed ring-C cleavage occurring between C-9 and 11 and C-8 and 14. This fragmentation, diagnostic of all hopanoids, also occurs in BHPs containing an amino group (-NH(2)) at C-35 although the higher relative stability of the ion limits this fragmentation to a minor process after protonation of the basic nitrogen function. Studies of a number of cell cultures including a prochlorophyte (Prochlorothrix hollandica) and a cyanobacterium (Chlorogloeopsis LA) demonstrate the power of this technique to detect composite BHPs with a complex biological functionality at C-35. We also report the first observation of intact pentafunctionalised bacteriohopanepolyols using this method.