Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]
Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]
复制标题
DOI：
10.1364/prj.1.000042
复制
发表时间：
2013-06
影响因子：
7.6
通讯作者：
X. Tsampoula;M. Mazilu;T. Vettenburg;F. Gunn-Moore;K. Dholakia
中科院分区：
文献类型：
--
作者：
X. Tsampoula;M. Mazilu;T. Vettenburg;F. Gunn-Moore;K. Dholakia
我们表明，超振荡光场，使用光学本征模的方法，使更有效的多光子介导的细胞转染。用质粒转染中国仓鼠卵巢细胞，并在线粒体中表现出DsRed-Mito的表达。我们证明了35%的效率提高相比，衍射限制点。这为纳米级局部细胞转染开辟了新的前景。
We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.