1',5'-anhydrohexitol oligonucleotides: Synthesis, base pairing and recognition by regular oligodeoxyribonucleotides and oligoribonucleotides
1',5'-anhydrohexitol oligonucleotides: Synthesis, base pairing and recognition by regular oligodeoxyribonucleotides and oligoribonucleotides
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DOI：
10.1002/chem.19970030118
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发表时间：
1997-01-01
影响因子：
4.3
通讯作者：
Herdewijn, P
中科院分区：
文献类型：
--
作者：
Hendrix, C;Rosemeyer, H;Herdewijn, P
由1 '，5'-脱水己糖醇核苷结构单元构建的寡核苷酸己糖醇核酸（hexitol nucleic acids，HNA）对3 ′-核酸外切酶是完全稳定的，并且与天然DNA和RNA形成非常稳定的自身互补双链体以及序列选择性稳定双链体，还观察到三螺旋形成，这些杂交特征高度依赖于碱基序列和实验条件，当使用含有0.1M NaCl的磷酸盐缓冲液时，高嘌呤HNA十二聚体与作为互补物的DNA产生+1.3 ℃/碱基对的Δ T-m，与作为互补物的RNA产生+3.0 ℃/碱基对的Δ T-m。因此，这些寡聚体作为反义构建体可能是相当有意义的。
Oligonucleotides constructed of 1',5'-anhydrohexitol nucleoside building blocks (hexitol nucleic acids, HNA) are completely stable towards 3'-exonuclease and form very stable self-complementary duplexes as well as sequence-selective stable duplexes with the natural DNA and RNA, Triple-helix formation has also been observed, These hybridisation characteristics are highly dependent on the base sequence and the experimental conditions, When using a phosphate buffer containing 0.1M NaCl, a homopurine HNA dodecamer gives a Delta T-m of +1.3 degrees C/ base pair with DNA as complement and a Delta T-m of +3.0 degrees C/base pair with RNA as complement, These oligomers may therefore be of considerable interest as antisense constructs.