Comparative genomic analysis of mitogen activated protein kinase gene family in grapevine
Comparative genomic analysis of mitogen activated protein kinase gene family in grapevine
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DOI：
10.1007/s13258-010-0010-0
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发表时间：
2010-06-01
期刊：
影响因子：
2.1
通讯作者：
Kim, Sun-Hyung
中科院分区：
文献类型：
--
作者：
Hyun, Tae Kyung;Kim, Ju-Sung;Kim, Sun-Hyung
丝裂原活化蛋白激酶（MAPKs）是参与细胞外信号转导到细胞内目标的重要蛋白，在生物和非生物应激反应中起着至关重要的作用。虽然拟南芥的MAPK被用于鉴定高等植物中推测的MAPK，但文献中尚未出现葡萄藤MAPK基因命名法。在本研究中，我们通过对现有葡萄基因组数据库的In- silicon分析，确定了葡萄MAPK基因（VvMPK）家族的12个成员。通过染色体位置分析、序列注释分析和同源基因对分析，对12个vvmpk的结构进行了比较，表明vvmpk是通过片段复制而不是串联扩增进化而来的。虽然还需要通过体内和体内实验进一步分析vmpk的功能，但我们的研究为未来研究mapk在葡萄中作用的多种信号通路提供了基础。
Mitogen activated protein kinases (MAPKs) are important proteins involved in the signal transduction of extracellular information to intracellular targets, and play a crucial role in the response to biotic and abiotic stresses. Although Arabidopsis MAPKs are used for identification of the putative MAPKs in higher plants, no grapevine MAPK gene nomenclature has yet been appeared in the literature. In this study, we have identified 12 members of grapevine MAPK gene (VvMPK) family via In-silico analysis of current grapevine genome database. The structural comparison of 12 VvMPKs through the analysis of chromosome locations, sequence annotation and paralogous gene pair indicated that VvMPKs have evolved by segmental duplication, rather than by tandem amplification. Although further functional analysis of VvMPKs through in vivo and in vivo experiments will be required, our study provides the basis for future research on the diverse signaling pathways medicated by MAPKs in grapevine.