Transcriptomic analysis reveals the regulation network of BmKrüppel homolog 1 in the oocyte development of Bombyx mori.
Transcriptomic analysis reveals the regulation network of BmKrüppel homolog 1 in the oocyte development of Bombyx mori.
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转录组分析揭示了 BmKrüppel 同源物 1 在家蚕卵母细胞发育中的调控网络。
DOI：
10.1111/1744-7917.12747
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发表时间：
2021
期刊：
影响因子：
4
通讯作者：
Huimin Deng
中科院分区：
文献类型：
--
作者：
Zidan Zhu;Qihao Hu;Chunmei Tong;Hongguang Yang;Sichun Zheng;Qili Feng;Huimin Deng
Krüppel同源物1（Kr‐h1）是一种锌指转录因子，参与昆虫的变态和成虫繁殖。然而，Kr ‐ h1在全代谢昆虫生殖中的作用仍有待阐明。本研究对家蚕生殖过程中Kr ‐h1‐相关基因的调控网络进行了研究。BmKr-h1在蛹后期和成虫卵巢中的表达水平较高。RNA干扰（RNAi）介导的BmKr-h1在发情第6天的雌性中的耗竭导致在双链RNA处理后48 h的异常卵母细胞，其显示较少的蛋黄蛋白沉积和部分透明的绒毛膜。RNA-seq和随后的差异表达转录本分析显示，在发育的第8天，BmKr-h1的敲低导致卵母细胞中2882个基因的表达减少，2565个基因的表达增加。共有27个编码转录因子的基因下调，而6个编码其他转录因子的基因上调。BmKr-h1与转录因子AP-1（激活蛋白-1）和FOXG 1的Kr-h1结合位点结合，分别增加BmN细胞中它们的信使RNA转录。对AP-1和FOXG 1共表达阳性的基因进行了基因本体富集和京都基因与基因组百科全书途径分析，结果显示富集主要集中在代谢相关途径、营养吸收和卵黄蛋白吸收过程中。这些数据表明BmKr ‐ h1可能直接调节代谢相关途径、营养吸收和卵黄蛋白吸收过程，或可能通过AP ‐ 1和/或FOXG 1调节卵母细胞发育。
Krüppel homolog 1(Kr‐h1), a zinc finger transcription factor, is involved in the metamorphosis and adult reproduction of insects. However, the role ofKr‐h1in reproduction of holometabolic insects remains to be elucidated. The regulation network ofKr‐h1‐associated genes in the reproduction inBombyx moriwas investigated in this study. The higher expression level ofBmKr‐h1in the ovaries was detected during the late pupal stage and adults. RNA interference (RNAi)‐mediated depletion ofBmKr‐h1in the female at day 6 of pupae resulted in abnormal oocytes at 48 h post‐double‐stranded RNA treatment, which showed less yolk protein deposition and partially transparent chorion. RNA‐seq and subsequent differentially expressed transcripts analysis showed that knockdown ofBmKr‐h1caused a decrease in the expression of 2882 genes and an increase in the expression of 2565 genes in the oocytes at day 8 of pupae. Totally, 27 genes coding for transcription factors were down‐regulated, while six genes coding for other transcription factors were up‐regulated. BmKr‐h1 bound to the Kr‐h1 binding site of the transcription factorsAP‐1(activating protein‐1) andFOXG1to increase their messenger RNA transcripts in the BmN cells, respectively. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses of that positively co‐expressed withAP‐1andFOXG1transcripts showed mainly enrichment in the metabolic‐related pathways, the nutrient absorption and the yolk protein absorption processes. These data suggested thatBmKr‐h1might directly regulate the metabolic‐related pathways, the nutrient absorption and the yolk protein absorption processes or probably throughAP‐1and /orFOXG1to regulate oocyte development.