GLUTAMATE UPTAKE BY CULTURED RAT HEPATOCYTES IS MEDIATED BY HORMONALLY INDUCIBLE, SODIUM-DEPENDENT TRANSPORT-SYSTEMS
GLUTAMATE UPTAKE BY CULTURED RAT HEPATOCYTES IS MEDIATED BY HORMONALLY INDUCIBLE, SODIUM-DEPENDENT TRANSPORT-SYSTEMS
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DOI：
10.1016/0014-5793(83)81024-2
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发表时间：
1983-01-01
期刊：
影响因子：
3.5
通讯作者：
MECKE, D
中科院分区：
文献类型：
--
作者：
GEBHARDT, R;MECKE, D
发现原代单层培养中大鼠肝细胞的谷氨酸摄取由Na+非依赖性转运系统和高亲和力和低亲和力的两种Na+依赖性转运系统介导。半胱氨酸和其他模型氨基酸的抑制研究排除了中性氨基酸转运系统A、ASC和N的参与，并揭示了Na+依赖性机构明确代表阴离子转运系统。与Na+非依赖性部分相比，分离肝细胞中谷氨酸的Na+依赖性摄取缓慢，但在培养期间自发增加。在地塞米松的存在下，在培养的第二天刺激约10倍。
Glutamate uptake by rat hepatocytes in primary monolayer culture was found to be mediated by a Na+‐independent and by two Na+‐dependent transport systems of high and low affinity. Inhibition studies with cysteate and other model amino acids rules out the participation of the neutral amino acid transport systems A, ASC, and N and revealed that the Na+‐dependent agencies represent unequivocally anionic transport systems. Na+‐dependent uptake of glutamate in isolated hepatocytes was slow compared to the Na+‐independent portion, but increased spontaneously during cultivation. In the presence of dexamethasone it was stimulated about 10‐fold at the second day of cultivation.