The role of the miR1976/CD105/integrin αvβ6 axis in vaginitis induced by Escherichia coli infection in mice
The role of the miR1976/CD105/integrin αvβ6 axis in vaginitis induced by Escherichia coli infection in mice
复制标题
miR1976/CD105/整合素αvβ6轴在大肠杆菌感染诱发小鼠阴道炎中的作用
DOI：
10.1038/s41598-019-50902-w
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发表时间：
2019-10-08
影响因子：
4.6
通讯作者：
Zeng, Xin
中科院分区：
文献类型：
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作者：
Jiang, Lisha;Zhang, Lingling;Zeng, Xin
阴道炎在女性中非常常见，尤其是育龄妇女，与早产和盆腔炎的风险显著增加有关。阴道植物群的不平衡是阴道炎的主要原因，它促进了阴道感染的发生和发展。然而，人们对负责机制仍然知之甚少。使用大肠杆菌感染的小鼠阴道炎模型，我们证明了microRNA 1976表达的降低和CD 105和整合素α v β 6表达的增加与阴道感染的进展密切相关。重要的是，我们首次证明了microRNA 1976/CD 105/整合素α v β 6轴调节E.大肠杆菌介导的小鼠阴道感染，这一发现证明，E。大肠杆菌诱导的阴道感染被microRNA 1976过表达逆转，并被CD 105过表达加重。microRNA 1976对CD 105和整联蛋白α v β 6的调节在腺病毒载体治疗的阴道炎小鼠模型中得到进一步证实。综上所述，我们的数据表明microRNA 1976负调控E.大肠杆菌诱导的小鼠阴道感染至少部分通过抑制CD 105和整合素α v β 6的表达。这些发现可能为E.大肠杆菌诱导的阴道炎，确定一种新的诊断生物标志物和潜在的治疗目标，为植物群不平衡相关的阴道炎。
Vaginitis is very common among women, especially women of childbearing age, and is associated with significantly increased risk of preterm birth and pelvic inflammatory diseases. An imbalance in the vaginal flora, the primary cause of vaginitis, promotes the initiation and progression of vaginal infections. However, the responsible mechanisms are still poorly understood. Using a murine vaginitis model of Escherichia coli infection, we demonstrated that decreased expression of microRNA1976 and increased expression of CD105 and integrin alpha v beta 6 were closely associated with the progression of vaginal infection. Importantly, we demonstrated for the first time that the microRNA1976/CD105/integrin alpha v beta 6 axis regulates E. coli-mediated vaginal infection in mice, as evidenced by the finding that E. coli-induced vaginal infection was reversed by microRNA1976 overexpression and exacerbated by CD105 overexpression. The regulation of CD105 and integrin alpha v beta 6 by microRNA1976 was further confirmed in a murine model of vaginitis with adenoviral vector treatment. Taken together, our data suggested that microRNA1976 negatively regulates E. coli-induced vaginal infection in mice at least in part by suppressing CD105 and integrin alpha v beta 6 expression. These findings may provide new insight into the mechanisms of E. coli-induced vaginitis, identify a novel diagnostic biomarker and a potential therapeutic target for flora imbalance-associated vaginitis.