Human antibody response to surface layer proteins in Clostridium difficile infection
Human antibody response to surface layer proteins in Clostridium difficile infection
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DOI：
10.1016/j.femsim.2004.03.007
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发表时间：
2004-07-01
影响因子：
--
通讯作者：
Kelly, CP
中科院分区：
文献类型：
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作者：
Drudy, D;Calabi, E;Kelly, CP
艰难梭菌是住院病人感染性腹泻的主要原因。表面层蛋白（SLPs）是难辨梭菌表达的最丰富的表面定位蛋白。本研究的目的是检查艰难梭菌slp的体液免疫反应及其在保护艰难梭菌相关性腹泻（CDAD）中的潜在作用。146例患者（55例CDAD患者，34例无症状携带者，57例对照组）采用ELISA检测艰难梭菌slp血清抗体。在检测的任何时间点，病例、携带者和对照组之间血清IgM、IgA或IgG抗体水平均无显著差异。然而，反复发作艰难梭菌腹泻患者在第1、3、6和9天的igm -anti - slp水平显著低于单次发作的患者（p = 0.05, p = 0.009, p = 0.021, p = 0.049）。第3天血清IgM抗slp抗体水平低与复发性腹泻相关的校正优势比为24.5（95%可信区间；1.6376.3）。进一步的研究将检验对定殖菌株的特异性抗slp抗体反应，以确定对艰难梭菌slp的免疫反应是否在预防CDAD中起作用。(C) 2004年欧洲微生物学会联合会。Elsevier B.V.版权所有。
Clostridium difficile is a major cause of infectious diarrhoea in hospitalised patients. Surface layer proteins (SLPs) are the most abundant surface localised proteins expressed by C difficile. The aim of this study was to examine the humoral immune response to C difficile SLPs and its potential role in protection from C difficile associated diarrhoea (CDAD). Serum antibodies to SLPs from C difficile were measured by ELISA in a cohort of 146 patients (55 patients with CDAD, 34 asymptomatic carriers, and 57 controls). No significant difference was detected in serum IgM, IgA or IgG antibody levels between cases, carriers or control groups at any of the time points tested. However, patients with recurrent episodes of C difficile diarrhoea had significantly lower IgM-antiSLP levels than patients with a single episode on days 1, 3, 6 and 9 (p = 0.05, p = 0.009, p = 0.021, p = 0.049). The adjusted odds ratio for recurrent diarrhoea associated with a low day 3 serum IgM anti-SLP antibody level was 24.5 (95% confidence interval; 1.6376.3). Further studies which examine the specific anti-SLP antibody responses to the colonising strain are warranted to determine if immune responses to C. difficile SLPs play a role in protection from CDAD. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.